Association of vascular endothelial growth factor A polymorphisms and aberrant expression of connexin 43 and VEGFA with idiopathic recurrent spontaneous miscarriage

Aim Idiopathic recurrent spontaneous miscarriage (IRSM) is one of the pregnancy outcomes that affects 1–2% of women trying to conceive. Specific genotype or aberrant expression of vascular endothelial growth factor A (VEGFA) and connexin 43 (Cx43) as two important genes for embryonic development are...

Full description

Saved in:
Bibliographic Details
Published in:The journal of obstetrics and gynaecology research 2020-03, Vol.46 (3), p.369-375
Main Authors: Sajjadi, Maryam S., Ghandil, Pegah, Shahbazian, Nahid, Saberi, Alihossein
Format: Article
Language:English
Subjects:
Abortion, Habitual - genetics
Abortion, Habitual - metabolism
Adult
angiogenesis
Case-Control Studies
Connexin 43
Connexin 43 - genetics
Connexin 43 - metabolism
Embryogenesis
Female
Fetuses
Gene polymorphism
Genotype
Genotyping
Haplotypes
Humans
idiopathic recurrent spontaneous miscarriage
Miscarriage
Polymorphism
Polymorphism, Single Nucleotide
Pregnancy
Restriction fragment length polymorphism
Vascular endothelial growth factor
vascular endothelial growth factor A
Vascular Endothelial Growth Factor A - genetics
Vascular Endothelial Growth Factor A - metabolism
Villus
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Aim Idiopathic recurrent spontaneous miscarriage (IRSM) is one of the pregnancy outcomes that affects 1–2% of women trying to conceive. Specific genotype or aberrant expression of vascular endothelial growth factor A (VEGFA) and connexin 43 (Cx43) as two important genes for embryonic development are deemed to increase the risk of IRSM. Methods To investigate any possible association of VEGFA polymorphisms and aberrant expression of Cx43 and VEGFA with IRSM, we carried out a case–control study including embryo chorionic villus tissues of 100 pregnant women with IRSM and 100 embryo chorionic villus tissues of healthy pregnant women without history of miscarriage. Restriction fragment length polymorphism was used for genotyping of rs699947 (−2578C/A) and rs2010963 (−634G/C) polymorphisms in VEGFA. Besides, quantitative real‐time PCR was performed for VEGFA and Cx43 expression analysis. Results The results showed that the frequency of −634G/C and C/C genotypes was significantly higher in aborted fetuses (P = 0.001 and P
ISSN:1341-8076
1447-0756
DOI:10.1111/jog.14192