Pinus residue/pectin-based composite hydrogels for the immobilization of β-D-galactosidase

• Published in: International journal of biological macromolecules 2020-04, Vol.149, p.773-782
• Main Authors: Cargnin, Mariana Aguiar, de Souza, Alana Gabrieli, de Lima, Giovanni Floriano, Gasparin, Bruna Carla, Rosa, Derval dos Santos, Paulino, Alexandre Tadeu
• Format: Article
• Language: English
• Subjects: beta-Galactosidase - chemistry; Enzyme Activation; Enzyme Stability; Enzymes, Immobilized - chemistry; Hydrogel; Hydrogels - chemistry; Hydrogen-Ion Concentration; Hydrolysis; Immobilization; Kinetics; Lactose - chemistry; Pectin; Pectins - chemistry; Pinus - chemistry; Pinus residue; Rheology; Spectroscopy, Fourier Transform Infrared; Temperature; Time Factors; Wood; X-Ray Diffraction; β-D-Galactosidase
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2020.01.280

The aim of this work was to synthesize pinus residue/pectin-based composite hydrogels for the immobilization of β-D-galactosidase. These hydrogels were synthesized via chemical crosslinking, and characterized by Fourier-transform infrared spectroscopy, scanning electron microscopy, thermal analysis, mechanical assays, X-ray diffraction, and swelling kinetics. The water absorption mechanism in the hydrogel networks occurs by non-Fickian transport. The β-D-galactosidase immobilization capacities of the hydrogels containing 0, 5 and 10% of pinus residue were respectively 242.08 ± 0.36, 181.27 ± 0.50 and 182.71 ± 0.36 mg enzyme per g dried hydrogel, at pH 4.0 and after 600 min. These values were 182.99 ± 0.41, 219.99 ± 0.47 and 218.56 ± 0.39 mg g−1, respectively, at pH 5.6. Pectin-based hydrogels demonstrated to be excellent solid supports for the immobilization of enzymes. β-D-Galactosidase immobilized in pectin-based hydrogels could be applied in the hydrolysis of lactose contained in either dairy foods or lactose-intolerant individuals. [Display omitted] •Pectin-based hydrogels were synthesized and characterized.•Wood fiber affects the crosslinking density and crystalline structure of hydrogels.•Pinus residue improved the mechanical and thermal resistances of hydrogels.•Pectin-based hydrogels are efficient supports for β-D-galactosidase immobilization.•β-D-Galactosidase immobilized in hydrogel can be used for the hydrolysis of lactose.