The Effect of Cold Atmospheric Plasma on the Membrane Permeability of Human Osteosarcoma Cells

Cold atmospheric plasma (CAP) has a variety of anticancer effects on different cancer cell types. In osteosarcoma (OS) cells, CAP reduces growth and motility, induces apoptosis, and alters secretion of cellular factors. The influence of CAP on membrane integrity of OS cells is unknown. Two different...

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Bibliographic Details
Published in:Anticancer research 2020-02, Vol.40 (2), p.841-846
Main Authors: Haralambiev, Lyubomir, Nitsch, Andreas, Einenkel, Rebekka, Muzzio, Damian O, Gelbrich, Nadine, Burchardt, Martin, Zygmunt, Marek, Ekkernkamp, Axel, Stope, Matthias B, Gümbel, Denis
Format: Article
Language:English
Subjects:
Anticancer properties
Apoptosis
Biomedical materials
Bone cancer
Cell Line, Tumor
Cell Membrane Permeability - drug effects
Fluorescein
Fluorescein diacetate
Humans
Low molecular weights
Membrane permeability
Membranes
Molecular weight
Osteosarcoma
Osteosarcoma - drug therapy
Osteosarcoma - pathology
Osteosarcoma cells
Permeability
Plasma Gases - pharmacology
Plasma Gases - therapeutic use
Sarcoma
Translocation
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Summary:Cold atmospheric plasma (CAP) has a variety of anticancer effects on different cancer cell types. In osteosarcoma (OS) cells, CAP reduces growth and motility, induces apoptosis, and alters secretion of cellular factors. The influence of CAP on membrane integrity of OS cells is unknown. Two different OS cell lines (U-2 OS and MNNG-HOS) were treated with CAP. Proliferation assays for cell growth after treatment was performed. Alterations in membrane permeability and the associated translocation of low molecular weight particles through the cytoplasmic membrane of OS cells after CAP treatment were shown in fluorescein diacetate (FDA) assays. FDA increasingly passed the membrane after CAP treatment and this effect depended on the duration of treatment. It was also shown that after CAP treatment, FDA was able to diffuse into the cells from the outside as well as out of the cell interior. These effects were observed when CAP-treated buffer was used and therefore no direct contact between cells and CAP occurred. The observations suggest that changes in membrane permeability and function may contribute to the antiproliferative effects of CAP.
ISSN:0250-7005
1791-7530
DOI:10.21873/anticanres.14016