Genetic diversity of group A rotaviruses in Moscow in 2018-2019

Here, we present the results of a study in which 639 samples obtained between October 2018 and April 2019 from patients with symptoms of acute gastroenteritis were tested for the presence of a rotavirus infection. The antigen of group A rotavirus was detected in 160 samples (25% of those tested). To...

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Bibliographic Details
Published in:Archives of virology 2020-03, Vol.165 (3), p.691-702
Main Authors: Ivashechkin, A. A., Yuzhakov, A. G., Grebennikova, T. V., Yuzhakova, K. A., Kulikova, N. Y., Kisteneva, L. B., Smetanina, S. V., Bazarova, M. V., Almazova, M. G.
Format: Article
Language:English
Subjects:
Adolescent
Adult
Amino Acid Sequence
Biomedical and Life Sciences
Biomedicine
Child
Child, Preschool
Gastroenteritis
Genetic diversity
Genetic Variation
Genomes
Genotype
Genotypes
Humans
Infant
Infectious Diseases
Medical Microbiology
Middle Aged
Moscow
Original Article
Phylogeny
Polymerase chain reaction
Reverse transcription
Ribonucleic acid
RNA
Rotavirus
Rotavirus - genetics
Rotavirus Infections - epidemiology
Rotavirus Infections - virology
Viral Proteins - chemistry
Viral Proteins - genetics
Viral Proteins - metabolism
Virology
Viruses
Young Adult
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Summary:Here, we present the results of a study in which 639 samples obtained between October 2018 and April 2019 from patients with symptoms of acute gastroenteritis were tested for the presence of a rotavirus infection. The antigen of group A rotavirus was detected in 160 samples (25% of those tested). To study the genetic diversity of group A rotavirus, RNA was isolated from the samples, and polymerase chain reaction combined with reverse transcription (RT-PCR) with primers specific for the VP4, VP6, and VP7 genes of group A rotaviruses was performed. At least one fragment of the group A rotavirus genome was found in 101 samples (15.8%). These fragments were sequenced, and their G and P genotypes—as well as their combinations—were determined. The predominant G genotypes were G9 (35.8% of all genotyped samples) and G4 (28.4%), but the rare G12 genotype was also found (3.0%). The dominant P genotype was P[8]. The spectrum of certain G/P combinations of genotypes included seven variants. The most common variants were G9P[8] (37.2%) and G4P[8] (30.2%).
ISSN:0304-8608
1432-8798
DOI:10.1007/s00705-020-04534-5