Enhancement of wound healing by chitosan/hyaluronan polyelectrolyte membrane loaded with glutathione: in vitro and in vivo evaluations

•Novel, chitosan/hyaluronan (Ch/HA) membranes loaded with glutathione have been prepared and characterized.•Glutathione loaded into the Ch/HA membrane showed great improvements in haemocompatibility, cytotoxicity and antioxidant properties.•In vivo experiments in the rat model showed a considerable...

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Bibliographic Details
Published in:Journal of biotechnology 2020-02, Vol.310, p.103-113
Main Authors: Tamer, Tamer M., Hassan, Mohamed A., Valachová, Katarína, Omer, Ahmed M., El-Shafeey, Muhammad E.A., Mohy Eldin, Mohamed S., Šoltés, Ladislav
Format: Article
Language:English
Subjects:
Polysaccharides
Rat model
Thiol compound
Wound dressing
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Summary:•Novel, chitosan/hyaluronan (Ch/HA) membranes loaded with glutathione have been prepared and characterized.•Glutathione loaded into the Ch/HA membrane showed great improvements in haemocompatibility, cytotoxicity and antioxidant properties.•In vivo experiments in the rat model showed a considerable increase in the wound healing process in the presence of glutathione. The inflammation of chronic wounds generally causes delaying their healing process. The present work aims to formulate a wound dressing polyelectrolyte membrane based on chitosan (Ch) and sodium hyaluronate (HA) loaded with glutathione (GSH). The membrane types (Ch/HA and Ch/HA/GSH) were examined by Fourier transform infrared spectroscopy (FT-IR). The material properties were further investigated using thermal gravimetric analysis (TGA) and scanning electron microscopy (SEM). Physical characteristics of the prepared membranes, such as wettability, surface roughness, and mechanical properties were determined by standard experimental methods. In vitro assays were used to evaluate the haemocompatibility, thrombogenicity, and cytotoxicity of the membranes. The wound healing examined using a standard rat model exhibited a progress at exploiting the Ch/HA/GSH-type membranes compared to a bicomponent Ch/HA membrane or a “dry” healing wound. Histological examination of the recovered skin confirmed the visual observations. In conclusion, in vivo study results assert that Ch/HA/GSH is a proper wound-dressing for healing the chronic skin wounds.
ISSN:0168-1656
1873-4863
DOI:10.1016/j.jbiotec.2020.02.002