Reparative effects of lycium barbarum polysaccharide on mouse ovarian injuries induced by repeated superovulation

To explore the repair effect of lycium barbarum polysaccharide (LBP) on ovarian injuries induced by repeated superovulation in mice, a model of ovarian injury was established, and ovarian repair was assessed after intragastric administration of LBP. The oocyte quality and blastocyst rates of pronucl...

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Published in:Theriogenology 2020-03, Vol.145, p.115-125
Main Authors: Liu, Bing, Wang, Jun-Li, Wang, Xin-Mei, Zhang, Chao, Dai, Jia-Ge, Huang, Xiao-Meng, Gao, Jian-Ming
Format: Article
Language:English
Subjects:
8-Hydroxy-2'-Deoxyguanosine - metabolism
Animals
Embryo, Mammalian
Female
In Vitro Oocyte Maturation Techniques
Injury model
LBP
Lipid Peroxidation
Lycium - chemistry
Mice
Oocytes
Ovary - drug effects
Ovary - injuries
Oxidative stress
Polysaccharides - chemistry
Polysaccharides - pharmacology
Remedy
Specific Pathogen-Free Organisms
Superovulation
Superovulatory times
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Summary:To explore the repair effect of lycium barbarum polysaccharide (LBP) on ovarian injuries induced by repeated superovulation in mice, a model of ovarian injury was established, and ovarian repair was assessed after intragastric administration of LBP. The oocyte quality and blastocyst rates of pronuclear embryos in vitro were observed. The levels of 8-hydroxydeoxyguanosine (8-OHdG) and lipid peroxide (LPO) in ovarian tissue were measured, and ovarian damage was assessed in paraffin sections. The groups with significant injury were selected according to the above observation, mice in the significant injury group were intragastrically administered with LBP (low dose, 25 mg/kg; medium dose, 35 mg/kg; and high dose, 45 mg/kg) for 30 days. The above measurements and anti-Müllerian hormone (AMH) expression were detected in the mouse ovaries and the breeding verification was carried out. Our results showed that repeated superovulation could cause mouse oocyte quality to drop, significant differences started from 4 superovulation events (P 
ISSN:0093-691X
1879-3231
DOI:10.1016/j.theriogenology.2020.01.048