Detection of Bartonella infection in pet dogs from Manila, the Philippines

•First identification of Bartonella henselae in pet dogs from the Philippines.•Study of two group of pet dogs: “healthy” (n = 60) and “Ehrlichia suspect” (N = 56).•Only dogs from the “Ehrlichia suspect” group were PCR positive for Bartonella.•Low seroprevalence (2.6%) for Bartonella henselae in pet...

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Bibliographic Details
Published in:Acta tropica 2020-05, Vol.205, p.105277-105277, Article 105277
Main Authors: Singer, Gar A., Loya, Felina P., Lapsley, Wallis D., Tobar, Bret Z., Carlos, Sixto, Carlos, Rhodora S., Carlos, Enrique T., Adao, Davin Edric V., Rivera, Windell L., Jaffe, David A., Mazet, Jonna A.K., Chomel, Bruno B.
Format: Article
Language:English
Subjects:
Bartonella henselae
Dogs
Dogs Philippines
PCR
Serology
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Summary:•First identification of Bartonella henselae in pet dogs from the Philippines.•Study of two group of pet dogs: “healthy” (n = 60) and “Ehrlichia suspect” (N = 56).•Only dogs from the “Ehrlichia suspect” group were PCR positive for Bartonella.•Low seroprevalence (2.6%) for Bartonella henselae in pet dogs, only in the “healthy” group (5%).•Higher overall Bartonella henselae PCR prevalence (11.2%), only in dogs with suspect tick exposure history ((23.2%). Dogs can be infected by a wide range of Bartonella spp., but studies regarding the prevalence of Bartonella infection in dogs in the Philippines have not been conducted. This study aimed at determining the prevalence of Bartonella infection in pets dogs from two veterinary clinics in Metro Manila, The Philippines, using both serology and polymerase chain reaction (PCR). Blood samples from 116 dogs from two different groups, one of 60 mainly “healthy dogs” and the other one of 56 dogs enrolled in a tick-borne disease suspect group, were tested for presence of B. henselae antibodies and to detect Bartonella DNA using primers specific for the citrate synthase gene. Seroprevalence for B. henselae was very low (2.6%), as the only three (5%) seropositive dogs (titer 1:64) where among the healthy pet dog group. Following subsequent sequencing, 13 samples, all from the tick-borne disease group, were determined positive for B. henselae (11.2%). This is the first study to report dog infection with B. henselae in the Philippines. [Display omitted]
ISSN:0001-706X
1873-6254
DOI:10.1016/j.actatropica.2019.105277