RMS2 Encoding a GDSL Lipase Mediates Lipid Homeostasis in Anthers to Determine Rice Male Fertility

Plant male gametogenesis is a coordinated effort involving both reproductive tissues and sporophytic tissues, in which lipid metabolism plays an essential role. Although GDSL esterases/lipases have been well known as key enzymes for many plant developmental processes and stress responses, their func...

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Published in:Plant physiology (Bethesda) 2020-04, Vol.182 (4), p.2047-2064
Main Authors: Zhao, Juan, Long, Tuan, Wang, Yifeng, Tong, Xiaohong, Tang, Jie, Li, Jinglin, Wang, Huimei, Tang, Liqun, Li, Zhiyong, Shu, Yazhou, Liu, Xixi, Li, Shufan, Liu, Hao, Li, Jialin, Wu, Yongzhong, Zhang, Jian
Format: Article
Language:English
Subjects:
Gene Expression Regulation, Plant - genetics
Gene Expression Regulation, Plant - physiology
Lipase - genetics
Lipase - metabolism
Oryza - genetics
Oryza - metabolism
Oryza - physiology
Plant Proteins - genetics
Plant Proteins - metabolism
Reproduction - genetics
Reproduction - physiology
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Summary:Plant male gametogenesis is a coordinated effort involving both reproductive tissues and sporophytic tissues, in which lipid metabolism plays an essential role. Although GDSL esterases/lipases have been well known as key enzymes for many plant developmental processes and stress responses, their functions in reproductive development remain unclear. Here, we report the identification of a ( ) mutant in rice ( ), which is completely male sterile due to the defects in tapetum degradation, cuticle formation in sporophytic tissues, and impaired exine and central vacuole development in pollen grains. was map-based cloned as an endoplasmic reticulum-localized GDSL lipase gene, which is predominantly transcribed during early anther development. In , a three-nucleotide deletion and one base substitution (TTGT to A) occurred within the GDSL domain, which reduced the lipid hydrolase activity of the resulting protein and led to significant changes in the content of 16 lipid components and numerous other metabolites, as revealed by a comparative metabolic analysis. Furthermore, is directly targeted by the male fertility regulators Undeveloped Tapetum1 and Persistent Tapetal Cell1 both in vitro and in vivo, suggesting that may serve as a key node in the rice male fertility regulatory network. These findings shed light on the function of GDSLs in reproductive development and provide a promising gene resource for hybrid rice breeding.
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.19.01487