MicroRNA-205-5p Promotes Unstable Atherosclerotic Plaque Formation In Vivo

• Published in: Cardiovascular drugs and therapy 2020-02, Vol.34 (1), p.25-39
• Main Authors: Meng, Xiandong, Yin, Jianjiao, Yu, Xinli, Guo, Yonggang
• Format: Article
• Language: English
• Subjects: Animal models; Animals; Aorta - metabolism; Aorta - pathology; Aortic Diseases - genetics; Aortic Diseases - metabolism; Aortic Diseases - pathology; Apolipoprotein E; Apoptosis; Arteries; Arteriosclerosis; Atherogenesis; Atherosclerosis; Atherosclerosis - genetics; Atherosclerosis - metabolism; Atherosclerosis - pathology; Bone growth; Bone marrow; Bone Marrow Transplantation; Cardiology; Case-Control Studies; Cholesterol; Cholesterol, HDL - blood; Computer simulation; Disease Models, Animal; Disease Progression; Efflux; Gene expression; High density lipoprotein; Humans; Inflammation; Lipid metabolism; Lipids; Macrophages; Macrophages - metabolism; Macrophages - pathology; Medicine; Medicine & Public Health; Membrane Microdomains - metabolism; Membrane Microdomains - pathology; Mice, Inbred C57BL; Mice, Knockout, ApoE; MicroRNAs; MicroRNAs - genetics; MicroRNAs - metabolism; miRNA; Original Article; Pathogenesis; Phenotype; Phenotypes; Plaque, Atherosclerotic; Plaques; Rupture, Spontaneous; Serum levels; Therapeutic applications; THP-1 Cells; Transplantation; Triglycerides; Triglycerides - blood
• ISSN: 0920-3206; 1573-7241
• DOI: 10.1007/s10557-020-06935-9

Purpose Atherosclerosis is a narrowing of the arteries caused by plaque buildup. MicroRNAs (miRNAs) have been proposed to participate in the pathogenesis of atherosclerosis. Here, we aimed to investigate miR-205-5p’s role in promoting atherosclerotic progression. Methods Knock-in (KI) mice with human/murine miR-205-5p within the murine host gene for miR-205 ( MIR205HG ) were crossed with apolipoprotein E knockout (Apoe −/− ) mice. This miR-205KI Apoe −/− murine model was employed to study the impact of miR-205-5p in Apoe −/− mice susceptible to atherosclerotic plaque formation. Results miR-205KI Apoe −/− mice developed larger, more unstable plaques relative to their Apoe −/− counterparts (0.45 vs. 0.26 mm 2 , P