Diabetic Retinopathy Screening Using a Gold Nanoparticle–Based Paper Strip Assay for the At-Home Detection of the Urinary Biomarker 8-Hydroxy-2′-Deoxyguanosine

• Published in: American journal of ophthalmology 2020-05, Vol.213, p.306-319
• Main Authors: Hainsworth, Dean P., Gangula, Abilash, Ghoshdastidar, Shreya, Kannan, Raghuraman, Upendran, Anandhi
• Format: Article
• Language: English
• Subjects: Artificial intelligence; Biomarkers; Diabetic retinopathy; Visual impairment
• ISSN: 0002-9394; 1879-1891
• DOI: 10.1016/j.ajo.2020.01.032

We sought to assess a smartphone-based, gold nanoparticle–based colorimetric lateral flow immunoassay paper sensor for quantifying urine 8-hydroxy-2′-deoxyguanosine (8-OHdG) as a biomarker for diabetic retinopathy (DR) screening. Paper strips incorporate gold nanoparticle–8-OHdG antibody conjugates that produce color changes that are proportional to urine 8-OHdG and that are discernible on a smartphone camera photograph. Paper strip accuracy, precision, and stability studies were performed with 8-OHdG solutions of varying concentrations. Urine was collected from 97 patients with diabetes who were receiving DR screening examinations, including 7-field fundus photographs. DR was graded by standard methods as either low risk (no or mild DR) or high risk (moderate or severe DR). Paper sensor assays were performed on urine samples from patients and 8-OHdG values were correlated with DR grades. The differences in 8-OHdG values between the low- and high-risk groups were analyzed for outliers to identify the threshold 8-OHdG value that would minimize false-negative results. Lateral flow immunoassay paper strips quantitatively measure 8-OHdG and were found to be accurate, precise, and stable. Average urine 8-OHdG concentrations in study patients were 22 ± 10 ng/mg of creatinine in the low-risk group and 55 ± 11 ng/mg of creatinine in the high-risk group. Screening cutoff values of 8-OHdG >50 ng/mg of creatinine or urine creatinine >1.5 mg minimized screen failures, with 91% sensitivity and 81% specificity. Urinary 8-OHdG is a useful biomarker to screen DR. Quantitative 8-OHdG detection with the lateral flow immunoassay paper sensor and smartphone camera demonstrates its potential in DR screening. NOTE: Publication of this article is sponsored by the American Ophthalmological Society.