An integrated microfluidic system for rapid, automatic and high-throughput staining of clinical tissue samples for diagnosis of ovarian cancer

Accurate cancer diagnostic methods are of urgent need. Since traditional immunohistochemistry (IHC)-based approaches, while reliable, are labor-intensive and require well-trained technicians, we developed an integrated microfluidic platform capable of labeling ovarian cancer biomarkers (i.e. aptamer...

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Bibliographic Details
Published in:Lab on a chip 2020-03, Vol.20 (6), p.1103-1109
Main Authors: Huang, Sheng-Po, Chuang, Yuan-Jhe, Lee, Wen-Bin, Tsai, Yi-Cheng, Lin, Chang-Ni, Hsu, Keng-Fu, Lee, Gwo-Bin
Format: Article
Language:English
Subjects:
Biomarkers
Cancer
Diagnostic software
Diagnostic systems
Fluorescence
Microfluidics
Ovarian cancer
Paraffins
Reagents
Staining
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Summary:Accurate cancer diagnostic methods are of urgent need. Since traditional immunohistochemistry (IHC)-based approaches, while reliable, are labor-intensive and require well-trained technicians, we developed an integrated microfluidic platform capable of labeling ovarian cancer biomarkers (i.e. aptamer) within formalin-fixed, paraffin embedded tissues via molecular probes. Both aptamer-based 1) fluorescent staining and 2) IHC staining of clinical tissue samples could be automated in the microfluidic system in only 2-3 h (40-50% faster than conventional approaches) with
ISSN:1473-0197
1473-0189
DOI:10.1039/c9lc00979e