In vitro antioxidant and antimicrobial activities, and in vivo anti-inflammatory activity of crude and fractionated PHNQs from sea urchin (Evechinus chloroticus)

•E. chloroticus polyhydroxyl-1,4-naphthoquinones were extracted and fractionated.•In vitro antioxidant activities were determined by ORAC, DPPH and FRAP assays.•E. chloroticus PHNQ extracts exhibited antimicrobial activity.•The extracts exhibited anti-inflammatory activity comparable to Celecoxib. T...

Full description

Saved in:
Bibliographic Details
Published in:Food chemistry 2020-06, Vol.316, p.126339-126339, Article 126339
Main Authors: Hou, Yakun, Carne, Alan, McConnell, Michelle, Bekhit, Adnan A., Mros, Sonya, Amagase, Kikuko, Bekhit, Alaa El-Din A.
Format: Article
Language:English
Subjects:
4-naphthoquinone (PHNQ)
Anti-inflammation
Anti-microbial
Antioxidant
Evechinus chloroticus
Polyhydroxyl-1
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c368t-780481a1833c988d53194b9a5ec62c46e07480118ac89221b615afbb5b8152b83
cites cdi_FETCH-LOGICAL-c368t-780481a1833c988d53194b9a5ec62c46e07480118ac89221b615afbb5b8152b83
container_end_page 126339
container_issue
container_start_page 126339
container_title Food chemistry
container_volume 316
creator Hou, Yakun
Carne, Alan
McConnell, Michelle
Bekhit, Adnan A.
Mros, Sonya
Amagase, Kikuko
Bekhit, Alaa El-Din A.
description •E. chloroticus polyhydroxyl-1,4-naphthoquinones were extracted and fractionated.•In vitro antioxidant activities were determined by ORAC, DPPH and FRAP assays.•E. chloroticus PHNQ extracts exhibited antimicrobial activity.•The extracts exhibited anti-inflammatory activity comparable to Celecoxib. This study investigated the bioactivities of polyhydroxyl-1,4-naphthoquinone (PHNQ) extracts from Evechinus chloroticus shell waste. PHNQs were extracted from E. chloroticus shells and spines using different solvents and the crude extracts were fractionated by HPLC. The antioxidant activity of the PHNQ extracts were evaluated by the DPPH, ABTS, ORAC and FRAP assays. Ethyl acetate was the best extraction solvent and spine extracts showed better antioxidant activity than shell extracts (p 
doi_str_mv 10.1016/j.foodchem.2020.126339
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2353584761</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S030881462030193X</els_id><sourcerecordid>2353584761</sourcerecordid><originalsourceid>FETCH-LOGICAL-c368t-780481a1833c988d53194b9a5ec62c46e07480118ac89221b615afbb5b8152b83</originalsourceid><addsrcrecordid>eNqFkd1u1DAQhS0EokvhFSpfFoks_ovj3IGqQitV_EhwbTn2ROtVEhfbWbGPw5vibLrccjXj4-_MyD4IXVGypYTK9_ttH4KzOxi3jLAiMsl5-wxtqGp41ZCGPUcbwomqFBXyAr1KaU9IIal6iS44I0I0RGzQn_sJH3yOAZsp-_Dbu1JL707n0dsYOm8GbGz2hfOQ3p1u_WI7rK7KT_1gxtHkEI9n8ohDj22cHZz4Pi56mEwGh7_dffmeihRGnMDgOdpdmXd9e4ClmRO2uyHEkL2d09vX6EVvhgRvnuol-vnp9sfNXfXw9fP9zceHynKpctUoIhQ1VHFuW6VczWkrutbUYCWzQgJphCKUKmNVyxjtJK1N33V1p2jNOsUv0fU69zGGXzOkrEefLAyDmSDMSTNe81qJRtKCyhUtv5NShF4_Rj-aeNSU6CUevdfnePQSj17jKcarpx1zN4L7ZzvnUYAPKwDlpQcPUSfrYbLgfASbtQv-fzv-Am1Dpm0</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2353584761</pqid></control><display><type>article</type><title>In vitro antioxidant and antimicrobial activities, and in vivo anti-inflammatory activity of crude and fractionated PHNQs from sea urchin (Evechinus chloroticus)</title><source>ScienceDirect Journals</source><creator>Hou, Yakun ; Carne, Alan ; McConnell, Michelle ; Bekhit, Adnan A. ; Mros, Sonya ; Amagase, Kikuko ; Bekhit, Alaa El-Din A.</creator><creatorcontrib>Hou, Yakun ; Carne, Alan ; McConnell, Michelle ; Bekhit, Adnan A. ; Mros, Sonya ; Amagase, Kikuko ; Bekhit, Alaa El-Din A.</creatorcontrib><description>•E. chloroticus polyhydroxyl-1,4-naphthoquinones were extracted and fractionated.•In vitro antioxidant activities were determined by ORAC, DPPH and FRAP assays.•E. chloroticus PHNQ extracts exhibited antimicrobial activity.•The extracts exhibited anti-inflammatory activity comparable to Celecoxib. This study investigated the bioactivities of polyhydroxyl-1,4-naphthoquinone (PHNQ) extracts from Evechinus chloroticus shell waste. PHNQs were extracted from E. chloroticus shells and spines using different solvents and the crude extracts were fractionated by HPLC. The antioxidant activity of the PHNQ extracts were evaluated by the DPPH, ABTS, ORAC and FRAP assays. Ethyl acetate was the best extraction solvent and spine extracts showed better antioxidant activity than shell extracts (p &lt; 0.05). The HPLC fraction containing spinochrome E showed the highest antioxidant activity (p &lt; 0.05). The Minimum Inhibitory Concentration of the PHNQ crude extracts and the HPLC fractions ranged from 250 to 2500 µg/mL depending on the PHNQ extract and microbial species tested. Treatment by PHNQ extracts resulted in alteration of the morphology of the microbial cell wall as observed by transmission electron microscopy. PHNQ extracts also exhibited anti-inflammatory activity in rats (ED50 = 8.26 ± 0.22 µg), comparable to that of Celecoxib (6.12 ± 0.18 µg).</description><identifier>ISSN: 0308-8146</identifier><identifier>EISSN: 1873-7072</identifier><identifier>DOI: 10.1016/j.foodchem.2020.126339</identifier><identifier>PMID: 32044704</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>4-naphthoquinone (PHNQ) ; Anti-inflammation ; Anti-microbial ; Antioxidant ; Evechinus chloroticus ; Polyhydroxyl-1</subject><ispartof>Food chemistry, 2020-06, Vol.316, p.126339-126339, Article 126339</ispartof><rights>2020 Elsevier Ltd</rights><rights>Copyright © 2020 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c368t-780481a1833c988d53194b9a5ec62c46e07480118ac89221b615afbb5b8152b83</citedby><cites>FETCH-LOGICAL-c368t-780481a1833c988d53194b9a5ec62c46e07480118ac89221b615afbb5b8152b83</cites><orcidid>0000-0002-2622-9538 ; 0000-0003-2569-8620</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32044704$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hou, Yakun</creatorcontrib><creatorcontrib>Carne, Alan</creatorcontrib><creatorcontrib>McConnell, Michelle</creatorcontrib><creatorcontrib>Bekhit, Adnan A.</creatorcontrib><creatorcontrib>Mros, Sonya</creatorcontrib><creatorcontrib>Amagase, Kikuko</creatorcontrib><creatorcontrib>Bekhit, Alaa El-Din A.</creatorcontrib><title>In vitro antioxidant and antimicrobial activities, and in vivo anti-inflammatory activity of crude and fractionated PHNQs from sea urchin (Evechinus chloroticus)</title><title>Food chemistry</title><addtitle>Food Chem</addtitle><description>•E. chloroticus polyhydroxyl-1,4-naphthoquinones were extracted and fractionated.•In vitro antioxidant activities were determined by ORAC, DPPH and FRAP assays.•E. chloroticus PHNQ extracts exhibited antimicrobial activity.•The extracts exhibited anti-inflammatory activity comparable to Celecoxib. This study investigated the bioactivities of polyhydroxyl-1,4-naphthoquinone (PHNQ) extracts from Evechinus chloroticus shell waste. PHNQs were extracted from E. chloroticus shells and spines using different solvents and the crude extracts were fractionated by HPLC. The antioxidant activity of the PHNQ extracts were evaluated by the DPPH, ABTS, ORAC and FRAP assays. Ethyl acetate was the best extraction solvent and spine extracts showed better antioxidant activity than shell extracts (p &lt; 0.05). The HPLC fraction containing spinochrome E showed the highest antioxidant activity (p &lt; 0.05). The Minimum Inhibitory Concentration of the PHNQ crude extracts and the HPLC fractions ranged from 250 to 2500 µg/mL depending on the PHNQ extract and microbial species tested. Treatment by PHNQ extracts resulted in alteration of the morphology of the microbial cell wall as observed by transmission electron microscopy. PHNQ extracts also exhibited anti-inflammatory activity in rats (ED50 = 8.26 ± 0.22 µg), comparable to that of Celecoxib (6.12 ± 0.18 µg).</description><subject>4-naphthoquinone (PHNQ)</subject><subject>Anti-inflammation</subject><subject>Anti-microbial</subject><subject>Antioxidant</subject><subject>Evechinus chloroticus</subject><subject>Polyhydroxyl-1</subject><issn>0308-8146</issn><issn>1873-7072</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNqFkd1u1DAQhS0EokvhFSpfFoks_ovj3IGqQitV_EhwbTn2ROtVEhfbWbGPw5vibLrccjXj4-_MyD4IXVGypYTK9_ttH4KzOxi3jLAiMsl5-wxtqGp41ZCGPUcbwomqFBXyAr1KaU9IIal6iS44I0I0RGzQn_sJH3yOAZsp-_Dbu1JL707n0dsYOm8GbGz2hfOQ3p1u_WI7rK7KT_1gxtHkEI9n8ohDj22cHZz4Pi56mEwGh7_dffmeihRGnMDgOdpdmXd9e4ClmRO2uyHEkL2d09vX6EVvhgRvnuol-vnp9sfNXfXw9fP9zceHynKpctUoIhQ1VHFuW6VczWkrutbUYCWzQgJphCKUKmNVyxjtJK1N33V1p2jNOsUv0fU69zGGXzOkrEefLAyDmSDMSTNe81qJRtKCyhUtv5NShF4_Rj-aeNSU6CUevdfnePQSj17jKcarpx1zN4L7ZzvnUYAPKwDlpQcPUSfrYbLgfASbtQv-fzv-Am1Dpm0</recordid><startdate>20200630</startdate><enddate>20200630</enddate><creator>Hou, Yakun</creator><creator>Carne, Alan</creator><creator>McConnell, Michelle</creator><creator>Bekhit, Adnan A.</creator><creator>Mros, Sonya</creator><creator>Amagase, Kikuko</creator><creator>Bekhit, Alaa El-Din A.</creator><general>Elsevier Ltd</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-2622-9538</orcidid><orcidid>https://orcid.org/0000-0003-2569-8620</orcidid></search><sort><creationdate>20200630</creationdate><title>In vitro antioxidant and antimicrobial activities, and in vivo anti-inflammatory activity of crude and fractionated PHNQs from sea urchin (Evechinus chloroticus)</title><author>Hou, Yakun ; Carne, Alan ; McConnell, Michelle ; Bekhit, Adnan A. ; Mros, Sonya ; Amagase, Kikuko ; Bekhit, Alaa El-Din A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c368t-780481a1833c988d53194b9a5ec62c46e07480118ac89221b615afbb5b8152b83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>4-naphthoquinone (PHNQ)</topic><topic>Anti-inflammation</topic><topic>Anti-microbial</topic><topic>Antioxidant</topic><topic>Evechinus chloroticus</topic><topic>Polyhydroxyl-1</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hou, Yakun</creatorcontrib><creatorcontrib>Carne, Alan</creatorcontrib><creatorcontrib>McConnell, Michelle</creatorcontrib><creatorcontrib>Bekhit, Adnan A.</creatorcontrib><creatorcontrib>Mros, Sonya</creatorcontrib><creatorcontrib>Amagase, Kikuko</creatorcontrib><creatorcontrib>Bekhit, Alaa El-Din A.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hou, Yakun</au><au>Carne, Alan</au><au>McConnell, Michelle</au><au>Bekhit, Adnan A.</au><au>Mros, Sonya</au><au>Amagase, Kikuko</au><au>Bekhit, Alaa El-Din A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro antioxidant and antimicrobial activities, and in vivo anti-inflammatory activity of crude and fractionated PHNQs from sea urchin (Evechinus chloroticus)</atitle><jtitle>Food chemistry</jtitle><addtitle>Food Chem</addtitle><date>2020-06-30</date><risdate>2020</risdate><volume>316</volume><spage>126339</spage><epage>126339</epage><pages>126339-126339</pages><artnum>126339</artnum><issn>0308-8146</issn><eissn>1873-7072</eissn><abstract>•E. chloroticus polyhydroxyl-1,4-naphthoquinones were extracted and fractionated.•In vitro antioxidant activities were determined by ORAC, DPPH and FRAP assays.•E. chloroticus PHNQ extracts exhibited antimicrobial activity.•The extracts exhibited anti-inflammatory activity comparable to Celecoxib. This study investigated the bioactivities of polyhydroxyl-1,4-naphthoquinone (PHNQ) extracts from Evechinus chloroticus shell waste. PHNQs were extracted from E. chloroticus shells and spines using different solvents and the crude extracts were fractionated by HPLC. The antioxidant activity of the PHNQ extracts were evaluated by the DPPH, ABTS, ORAC and FRAP assays. Ethyl acetate was the best extraction solvent and spine extracts showed better antioxidant activity than shell extracts (p &lt; 0.05). The HPLC fraction containing spinochrome E showed the highest antioxidant activity (p &lt; 0.05). The Minimum Inhibitory Concentration of the PHNQ crude extracts and the HPLC fractions ranged from 250 to 2500 µg/mL depending on the PHNQ extract and microbial species tested. Treatment by PHNQ extracts resulted in alteration of the morphology of the microbial cell wall as observed by transmission electron microscopy. PHNQ extracts also exhibited anti-inflammatory activity in rats (ED50 = 8.26 ± 0.22 µg), comparable to that of Celecoxib (6.12 ± 0.18 µg).</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>32044704</pmid><doi>10.1016/j.foodchem.2020.126339</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-2622-9538</orcidid><orcidid>https://orcid.org/0000-0003-2569-8620</orcidid></addata></record>
fulltext fulltext
identifier ISSN: 0308-8146
ispartof Food chemistry, 2020-06, Vol.316, p.126339-126339, Article 126339
issn 0308-8146
1873-7072
language eng
recordid cdi_proquest_miscellaneous_2353584761
source ScienceDirect Journals
subjects 4-naphthoquinone (PHNQ)
Anti-inflammation
Anti-microbial
Antioxidant
Evechinus chloroticus
Polyhydroxyl-1
title In vitro antioxidant and antimicrobial activities, and in vivo anti-inflammatory activity of crude and fractionated PHNQs from sea urchin (Evechinus chloroticus)
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T02%3A45%3A05IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=In%20vitro%20antioxidant%20and%20antimicrobial%20activities,%20and%20in%20vivo%20anti-inflammatory%20activity%20of%20crude%20and%20fractionated%20PHNQs%20from%20sea%20urchin%20(Evechinus%20chloroticus)&rft.jtitle=Food%20chemistry&rft.au=Hou,%20Yakun&rft.date=2020-06-30&rft.volume=316&rft.spage=126339&rft.epage=126339&rft.pages=126339-126339&rft.artnum=126339&rft.issn=0308-8146&rft.eissn=1873-7072&rft_id=info:doi/10.1016/j.foodchem.2020.126339&rft_dat=%3Cproquest_cross%3E2353584761%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c368t-780481a1833c988d53194b9a5ec62c46e07480118ac89221b615afbb5b8152b83%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2353584761&rft_id=info:pmid/32044704&rfr_iscdi=true