Antimicrobial and antibiofilm activities of ursolic acid against carbapenem-resistant Klebsiella pneumoniae

Previous studies demonstrated that ursolic acid (UA) present in apple pomace displays antimicrobial activity against some microorganisms, but the underlying mechanisms associated with this activity remain unexplored. Furthermore, there are no reports on the effect of UA on carbapenem-resistant Klebs...

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Bibliographic Details
Published in:Journal of antibiotics 2020-06, Vol.73 (6), p.382-391
Main Authors: Qian, Weidong, Wang, Wenjing, Zhang, Jianing, Wang, Ting, Liu, Miao, Yang, Min, Sun, Zhaohuan, Li, Xiang, Li, Yongdong
Format: Article
Language:English
Subjects:
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14/19
14/28
38/77
631/326/1320
631/326/46
Adenosine Triphosphate - metabolism
Anti-Bacterial Agents - administration & dosage
Anti-Bacterial Agents - pharmacology
Antibiotics
Antimicrobial agents
Bacteriology
Biofilms
Biofilms - drug effects
Biomedical and Life Sciences
Bioorganic Chemistry
Carbapenem-Resistant Enterobacteriaceae - drug effects
Cell Membrane - drug effects
Gene expression
Humans
Hydrogen-Ion Concentration
Klebsiella pneumoniae
Klebsiella pneumoniae - drug effects
Life Sciences
Medicinal Chemistry
Microbial Sensitivity Tests
Microbiology
Microorganisms
Mode of action
Organic Chemistry
Triterpenes - administration & dosage
Triterpenes - pharmacology
Ursolic Acid
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Summary:Previous studies demonstrated that ursolic acid (UA) present in apple pomace displays antimicrobial activity against some microorganisms, but the underlying mechanisms associated with this activity remain unexplored. Furthermore, there are no reports on the effect of UA on carbapenem-resistant Klebsiella pneumoniae (CRKP). This study examined the antimicrobial activity and mode of action of UA against CRKP was examined. Minimum inhibitory concentration (MIC) of UA against CRKP was determined by the agar dilution method. Variations in the intracellular pH (pHin), ATP concentration, and cell membrane potential were measured to assess the influence of UA on the cell membrane. Our results show that UA was effective against CRKP at an MIC of 0.8 mg ml −1 . UA disrupted the cell membrane integrity of CRKP, exhibited strong inhibitory effects against biofilm formation and biofilm-related gene expression, and inactivated CRKP cells encased in biofilms. Thus, UA shows promise for use in combination with other antibiotics to treat multidrug resistant K. pneumoniae infections.
ISSN:0021-8820
1881-1469
DOI:10.1038/s41429-020-0285-6