Amino acids are key substrates to Escherichia coli BW25113 for achieving high specific growth rate

Studying substrate consumption in nutrient-rich conditions is challenging because often the growth medium includes undefined components like yeast extract or peptone. For clear and consistent results, it is necessary to use defined medium, where substrate utilization can be followed. In the present...

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Bibliographic Details
Published in:Research in microbiology 2020-07, Vol.171 (5-6), p.185-193
Main Authors: Maser, Andres, Peebo, Karl, Vilu, Raivo, Nahku, Ranno
Format: Article
Language:English
Subjects:
Acetates - metabolism
Amino acid utilization
Amino Acids - metabolism
Carbon - metabolism
Citric Acid Cycle
Culture Media
Defined nutrient-rich medium
Energy Metabolism
Escherichia coli
Escherichia coli - genetics
Escherichia coli - growth & development
Escherichia coli - metabolism
Escherichia coli Proteins - biosynthesis
Escherichia coli Proteins - chemistry
Fast growth
Gene Expression Regulation, Bacterial
Glucose - metabolism
Proteome
Proteome - analysis
Serine
Serine - metabolism
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Summary:Studying substrate consumption in nutrient-rich conditions is challenging because often the growth medium includes undefined components like yeast extract or peptone. For clear and consistent results, it is necessary to use defined medium, where substrate utilization can be followed. In the present work, Escherichia coli BW25113 batch growth in a medium supplemented with 20 proteinogenic amino acids and glucose was studied. Focus was on the quantitative differences in substrate consumption and proteome composition between minimal and nutrient-rich medium. In the latter, 72% of carbon used for biomass growth came from amino acids and 28% from glucose. Serine was identified as the most consumed substrate with 41% of total carbon consumption. Proteome comparison between nutrient-rich and minimal medium revealed changes in TCA cycle and acetate producing enzymes that together with extracellular metabolite data pointed to serine being consumed mainly for energy generation purposes. Serine removal from the growth medium decreased specific growth rate by 22%. In addition, proteome comparison between media revealed a large shift in amino acid synthesis and translation related proteins. Overall, this work describes in quantitative terms the batch growth carbon uptake profile and proteome allocation of E. coli BW25113 in minimal and nutrient-rich medium.
ISSN:0923-2508
1769-7123
DOI:10.1016/j.resmic.2020.02.001