Canine vector-borne disease in domestic dogs on Isla Santa Cruz, Galápagos

• Published in: Veterinary parasitology (Amsterdam) 2020-01, Vol.19, p.100373-100373, Article 100373
• Main Authors: Jimenez, Isabel Angelica, Vega Mariño, Patricio Alejandro, Stapleton, G. Sean, Prieto, Jennifer Battista, Bowman, Dwight Douglas
• Format: Article
• Language: English
• Subjects: Anaplasma - isolation & purification; Anaplasma phagocytophilum - isolation & purification; Anaplasmosis - epidemiology; Anaplasmosis - microbiology; Animals; Borrelia burgdorferi - isolation & purification; Dirofilaria immitis; Dog Diseases - epidemiology; Dog Diseases - microbiology; Dogs; Ecuador - epidemiology; Ehrlichia - isolation & purification; Ehrlichia canis; Ehrlichia canis - isolation & purification; Ehrlichiosis - epidemiology; Ehrlichiosis - microbiology; Ehrlichiosis - veterinary; Heartworm; Lyme Disease - epidemiology; Lyme Disease - microbiology; Lyme Disease - veterinary; Mosquito; Prevalence; Rhipicephalus sanguineus; Seroepidemiologic Studies; Tick
• ISSN: 2405-9390; 2405-9390
• DOI: 10.1016/j.vprsr.2020.100373

Vector-borne diseases result in significant morbidity and mortality in domestic dogs in tropical and subtropical regions and also pose a potential threat to wildlife species and humans. Ehrlichia canis, the causative agent of canine monocytic ehrlichiosis (CME), has a high reported seroprevalence in dogs on Santa Cruz in the Galápagos Islands, Ecuador. Veterinary diagnostic and treatment resources are often scarce and clinical follow-up is lacking in the Galápagos. This study evaluated 58 dogs presenting to the Darwin Animal Doctors clinic in the city of Puerto Ayora on Santa Cruz Island during August of 2018. The seroprevalence of E. canis/Ehrlichia ewingii (48.3%), Anaplasma phagocytophilum/Anaplasma platys (12.1%), and Borrelia burgdorferi (0%), as well as the proportion of dogs actively infected with E. canis (12.1%) and E. ewingii (0%), are reported. Active infection was defined as the identification of antigen by PCR. Dogs with a packed cell volume (PCV) ≤ 30% had a 10-fold risk of active infection with E. canis compared to dogs with a PCV ≥ 31% (p = .0124). A PCV cutoff of 30% may be a useful screening tool for active E. canis infection in regions with high Ehrlichia seroprevalence, in the absence of other apparent causes of anemia. Dirofilaria immitis antigen was present in 6.9% of examined dogs, with the highest prevalence in the barrio Las Ninfas. PCR and Sanger sequencing were used to provide the first molecular identification of D. immitis in the Galápagos. This study updates the seropositivity and prevalence data of these canine vector-borne pathogens and highlights the need for continued surveillance in the region. •This study documents the first report of Dirofilaria immitis in dogs on Santa Cruz.•This study reports seroprevalence of Ehrlichia (48.3%) and Anaplasma (12.1%).•This study reports the proportion of active infection with E. canis (12.1%).•Dogs with PCV ≤ 30% had a 10-fold risk of active Ehrlichia infection (p = .0124).