Genetic diversity and distribution of Fasciola hepatica haplotypes in Iran: Molecular and phylogenetic studies

Fasciolosis is a zoonotic disease caused by Fasciola hepatica and Fasciola gigantica. Over the last decade, diagnostic tools to detect and differentiate Fasciola species have improved, but our knowledge of the distribution of haplotypes and gene flow of this parasite is not comprehensive yet. The pu...

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Bibliographic Details
Published in:Veterinary parasitology (Amsterdam) 2020-01, Vol.19, p.100359-100359, Article 100359
Main Authors: Bozorgomid, Arezoo, Rouhani, Soheila, Harandi, Majid Fasihi, Ichikawa-Seki, Madoka, Raeghi, Saber
Format: Article
Language:English
Subjects:
Animals
Cattle
Cattle Diseases - parasitology
Fasciola hepatica
Fasciola hepatica - genetics
Fasciola hepatica - physiology
Fascioliasis - parasitology
Fascioliasis - veterinary
Genetic diversity
Genetic Variation
Goat Diseases - parasitology
Goats
Haplotypes
Helminth Proteins - genetics
Iran
NADH Dehydrogenase - genetics
ND1 gene
Sheep
Sheep Diseases - parasitology
Sheep, Domestic
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Summary:Fasciolosis is a zoonotic disease caused by Fasciola hepatica and Fasciola gigantica. Over the last decade, diagnostic tools to detect and differentiate Fasciola species have improved, but our knowledge of the distribution of haplotypes and gene flow of this parasite is not comprehensive yet. The purpose of this study was to investigate this gap in the epidemiology of F. hepatica in different provinces of Iran between 2015 and 2017. Isolated Fasciola were collected from abattoirs in 9 provinces. The partial sequence of mitochondrial NADH dehydrogenase subunit 1 (ND1) gene was used for the identification and molecular analysis of F. hepatica isolates. The amplified PCR products were purified and subjected to direct sequencing for subsequent construction of phylogenetic tree and network analysis. In the 130 subjects analyzed, 37 ND1 haplotypes were detected. This is the first study in Iran which investigates F. hepatica population and its genetic structure, based on mitochondrial ND1 marker in different geographical regions of Iran.
ISSN:2405-9390
2405-9390
DOI:10.1016/j.vprsr.2019.100359