Characterization and anti-diabetic effects of the oligosaccharide fraction isolated from Rosa canina in STZ-Induced diabetic rats

• Published in: Carbohydrate research 2020-03, Vol.489, p.107927-107927, Article 107927
• Main Authors: Rahimi, Mehrali, Sajadimajd, Soraya, Mahdian, Zahra, Hemmati, Marayam, Malekkhatabi, Peyman, Bahrami, Gholamreza, Mohammadi, Bahareh, Miraghaee, Shahram, Hatami, Razieh, Mansouri, Kamran, Moahammadi Motlagh, Hamid Reza, Keshavarzi, Samira, Derakhshankhah, Hossein
• Format: Article
• Language: English
• Subjects: Diabetes; Oligosaccharide; Regeneration; Rosa canina
• ISSN: 0008-6215; 1873-426X
• DOI: 10.1016/j.carres.2020.107927

Diabetes mellitus is the most common metabolic disorder characterized by chronic hyperglycemia. There has been a surge of research studies aiming to use natural products in the management of diabetes. The objective of this study was to isolate and characterize the structure and anti-diabetic mechanisms of the main ingredient from Rosa canina. The oligosaccharide was isolated from Rosa canina fruits and characterized by a combination of FTIR, NMR and Mass spectrometry. Wistar rats were divided into negative control, diabetic (type 2), isolated oligosaccharide (IO)-treated diabetic and positive diabetic controls. Oral glucose tolerance, gluconeogenesis and α-glucosidase inhibitory tests as well as immunohistochemistry and quantitative real time-PCR were performed to elucidate the molecular anti-diabetic mechanisms of IO. Structural analyses confirmed the oligosaccharide structure of isolated fraction. Gluconeogenesis and α-glucosidase activity were inhibited by IO in diabetic rats. The oral glucose tolerance test was improved significantly in the group treated with the IO (P