A convenient new method for reproducible fed-batch fermentation of fission yeast Schizosaccharomyces pombe

Objectives Development of an open-loop fed-batch protocol for highly reproducible fermentation of fission yeast that starts from batch cultures instead of glucose-limited aerobic chemostat cultures. Results A new strategy was employed that consists of an exponential feeding phase followed by a starv...

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Bibliographic Details
Published in:Biotechnology letters 2020-06, Vol.42 (6), p.937-943
Main Authors: Scomparin, Alberto, Bureik, Matthias
Format: Article
Language:English
Subjects:
Applied Microbiology
Batch Cell Culture Techniques - methods
Batch culture
Biochemistry
Biomass
Biomedical and Life Sciences
Biotechnology
Carbon dioxide
Carbon Dioxide - analysis
Carbon Dioxide - metabolism
Consumption
Feeding
Fermentation
Fermentation - physiology
Fission
Glucose
Glucose - analysis
Glucose - metabolism
Life Sciences
Microbiology
Original Research Paper
Process parameters
Schizosaccharomyces - cytology
Schizosaccharomyces - metabolism
Starvation
Yeast
Yeasts
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Summary:Objectives Development of an open-loop fed-batch protocol for highly reproducible fermentation of fission yeast that starts from batch cultures instead of glucose-limited aerobic chemostat cultures. Results A new strategy was employed that consists of an exponential feeding phase followed by a starvation period and then a linear feeding phase. A comparison of several independent fed-batch fermentations of a recombinant fission yeast strain showed that while during the initial phase process parameters such as glucose consumption and CO 2 evolution varied considerably as expected, they were much more uniform during the third phase. For instance, the normalized standard deviation of glucose consumption was thirty times higher during the exponential feeding phase of the fermentation that during the linear feeding phase. Conclusion These data demonstrate the usefulness of the proposed strategy. It is expected that by variation of only two parameters (the total amount of glucose fed in the initial phase and the time frame of the starvation phase) the protocol can easily be adapted to other microbes.
ISSN:0141-5492
1573-6776
DOI:10.1007/s10529-020-02840-1