Abundance of human pathogen genes in the phyllosphere of four landscape plants

• Published in: Journal of environmental management 2020-02, Vol.255, p.109933-109933, Article 109933
• Main Authors: Zhao, Dandan, Liu, Guijia, Wang, Xuefei, Daraz, Umar, Sun, Qingye
• Format: Article
• Language: English
• Subjects: Bacteria; Human pathogenic genes; Humans; Landscape plants; Microbiota; Phyllosphere bacteria; Plant Leaves; Plants; qPCR; RNA, Ribosomal, 16S; Staphylococcus aureus
• ISSN: 0301-4797; 1095-8630
• DOI: 10.1016/j.jenvman.2019.109933

The surface of leaf, also known as phyllosphere, harbors diverse microbial communities which include both beneficial microorganisms promoting plants growth and harmful microorganisms, such as plant pathogens and human pathogens. Several studies have investigated the interaction between plants and human pathogens, while few works have focused on the quantitative analysis of pathogenic bacteria. On the basis of real-time polymerase chain reaction (qPCR), this study aimed to evaluate the abundance of following genes: the nuc and pvl of Staphylococcus aureus, the lytA and psaA of Streptococcus pneumoniae, and the ttr and invA of Salmonella enterica in the phyllosphere of four landscape plants (Nandina domestica, Rhododendron pulchrum, Photinia serrulata, and Cinnamomum camphora) growing in two habitats. Our results indicated that the relative abundance of pathogenic genes in the phyllosphere ranged from 10−9 to 10−6. The specific genes of S. aureus, S. pneumoniae and S. enterica in landscape plants were pvl, lytA and ttr, respectively. The two pathogenic genes of S. pneumoniae and the 16S rRNA gene were mainly affected by habitats, host species, and habitats-species interaction. Moreover, for the abundance of lytA and 16S rRNA, results showed that plants present in roadside with traffic pollution were relatively higher than that of campus with less pollution. The N. domestica and C. camphora were recommended for planting along the roadsides due to lower abundance of pathogenic genes. However, we have observed no significant difference in the abundance of pathogenic genes among four plants in the campus. Thereby, this study provided a valuable reference for selecting landscape plants in view of human health. •Three human pathogens in phyllosphere of landscape plants were detected based on qPCR.•The abundance of pathogenic gene of human in phyllosphere is low.•Habitats, host species, and habitats-species interaction drive the abundance of pathogenic gene.