Schizandrin attenuates inflammation induced by avian pathogenic Escherichia coli in chicken type II pneumocytes

• Published in: International immunopharmacology 2020-04, Vol.81, p.106313-106313, Article 106313
• Main Authors: Yuan, Meng, Peng, Lu-Yuan, Wu, Shuai-cheng, Li, Jing-He, Song, Ke, Chen, Shuang, Huang, Jiang-Ni, Yu, Jia-Lin, An, Qiang, Yi, Peng-Fei, Shen, Hai-Qing, Fu, Ben-Dong
• Format: Article
• Language: English
• Subjects: Activation; Adhesion; Alveolar Epithelial Cells - physiology; Animals; Anti-Inflammatory Agents - therapeutic use; Avian pathogenic Escherichia coli; Bacteria; Bacterial Adhesion; Bacterial diseases; Cells, Cultured; Chicken Type II Pneumocytes; Chickens; Colibacillosis; Cyclooctanes - therapeutic use; Cytokines - metabolism; Drug resistance; E coli; Escherichia coli; Escherichia coli - physiology; Escherichia coli Infections - drug therapy; Extracellular Signal-Regulated MAP Kinases - metabolism; Gene expression; IL-1β; Inflammation; Inflammation - drug therapy; Inflammation Mediators - metabolism; Injury prevention; Interleukin 6; Interleukin 8; Kinases; L-Lactate dehydrogenase; L-Lactate Dehydrogenase - metabolism; Lactate dehydrogenase; Lactic acid; Lignans - therapeutic use; MAP kinase; MAPK; NF-kappa B - metabolism; NF-κB protein; Pili; Pneumocytes; Polycyclic Compounds - therapeutic use; Poultry; Poultry Diseases - drug therapy; Protein kinase; Schizandrin; Signal Transduction; Signaling; Tumor necrosis factor-TNF; Tumor necrosis factor-α; Virulence
• ISSN: 1567-5769; 1878-1705
• DOI: 10.1016/j.intimp.2020.106313

•Schizandrin inhibited the expression level of APEC-O78-associated virulence genes.•Schizandrin reduced the release of LDH from chicken type II pneumocytes.•Schizandrin inhibited the adhesion of APEC-O78 on chicken type II pneumocytes.•Schizandrin reduced the production of pro-inflammatory cytokines.•Schizandrin inhibited the MAPK signaling pathway and NF-κB signaling pathway. Avian pathogenic Escherichia coli (APEC) is a kind of highly pathogenic parenteral bacteria, which adheres to chicken type II pneumocytes through pili, causing inflammatory damage of chicken type II pneumocytes. Without affecting the growth of bacteria, anti-adhesion to achieve anti-inflammatory effect is considered to be a new method for the treatment of multi-drug-resistant bacterial infections. In this study, the anti-APEC activity of schizandrin was studied in vitro. By establishing the model of chicken type II pneumocytes infected with APEC-O78, the adhesion number, the expression of virulence genes, the release of lactate dehydrogenase (LDH), levels of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, IL-8 and activation of nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways were detected. The results showed that schizandrin reduced the release of LDH and the adherence of APEC on chicken type II pneumocytes. Moreover, schizandrin markedly decreased the levels of IL-1β, IL-8, IL-6, and TNF-α, the mechanism responsible for these effects was attributed to the inhibitory effect of schizandrin on NF-κB and MAPK signaling activation. In conclusion, our findings revealed that schizandrin could reduce the inflammatory injury of chicken type II pneumocytes by reducing the adhesion of APEC-O78 to chicken type II pneumocytes. The results indicate that schizandrin can be a potential agent to treat inflammation caused by avian colibacillosis.