Agarose native gel electrophoresis for characterization of antibodies

This study was conducted to evaluate applicability of the previously reported native agarose gel electrophoresis to the analysis of various monoclonal and polyclonal antibodies. Experiments were carried to test the electrophoresis system for characterization of different monoclonal antibodies and an...

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Bibliographic Details
Published in:International journal of biological macromolecules 2020-05, Vol.151, p.885-890
Main Authors: Li, Cynthia, Akuta, Teruo, Nakagawa, Masataka, Sato, Tomomi, Shibata, Takashi, Maruyama, Toshiaki, Okumura, C.J., Kurosawa, Yasunori, Arakawa, Tsutomu
Format: Article
Language:English
Subjects:
Agarose
Antibodies, Monoclonal - chemistry
Antibodies, Monoclonal - isolation & purification
Basic protein
Electrophoresis, Agar Gel
Expression
Histidine
Molecular Weight
Monoclonal antibody
Native electrophoresis
Protein Stability
Recombinant Proteins - chemistry
Sepharose
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Description
Summary:This study was conducted to evaluate applicability of the previously reported native agarose gel electrophoresis to the analysis of various monoclonal and polyclonal antibodies. Experiments were carried to test the electrophoresis system for characterization of different monoclonal antibodies and animal serum, analysis of expressed antibodies in cell culture and evaluation of antibody stability. An attempt to optimize the electrophoretic condition was made by adjusting the electrode buffer concentration, electrophoretic run time and agarose concentration.
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2020.02.185