A polysaccharide from Umbilicaria yunnana: Structural characterization and anti-inflammation effects

A polysaccharide JUYP was isolated and purified from Umbilicaria yunnana. The detailed structure of JUYP was studied using gas chromatography (GC), Fourier transform infrared spectroscopy (FTIR), methylation-GC–MS, nuclear magnetic resonance (NMR) and transmission electron microscopy (TEM). A homoge...

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Bibliographic Details
Published in:International journal of biological macromolecules 2020-05, Vol.151, p.870-877
Main Authors: Luo, Dianhui, Wang, Zhaojing, Zhou, Rong, Cao, Sen
Format: Article
Language:English
Subjects:
Animals
Anti-inflammation effects
Anti-Inflammatory Agents - chemistry
Anti-Inflammatory Agents - pharmacology
Ascomycota - chemistry
Chemical Phenomena
Cytokines - metabolism
Fungal Polysaccharides - chemistry
Fungal Polysaccharides - pharmacology
Gas Chromatography-Mass Spectrometry
Hydrolysis
Inflammation Mediators - metabolism
Methylation
Mice
Molecular Structure
Oxidation-Reduction
RAW 264.7 Cells
Spectrum Analysis
Structural characterization
Structure-Activity Relationship
Umbilicaria yunnana
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Summary:A polysaccharide JUYP was isolated and purified from Umbilicaria yunnana. The detailed structure of JUYP was studied using gas chromatography (GC), Fourier transform infrared spectroscopy (FTIR), methylation-GC–MS, nuclear magnetic resonance (NMR) and transmission electron microscopy (TEM). A homogeneous polysaccharide JUYP was obtained with the yield of 21.2% and average molecular weight (Mw) of 577 kDa. Monosaccharide composition analysis indicated that JUYP was composed of glucose, galactose and mannose with a molar ratio of 2.3:1:0.7. Structural analyses demonstrated that the dominate components in JUYP were 6-β-D-Glcp, and other sugar residues included 2,4-β-D-Manp and T-β-D-Galf. TEM images further revealed JUYP was a linear branched molecule with entangled chains. Based on the anti-inflammatory assays, 1 μg/mL of JUYP exhibited good inhibitory effects on TNF-α, IL-6, IL-1β and COX-2 mRNA expressions in LPS-stimulated RAW 264.7 cells, while the inhibitory effects (87.8% for mRNA, 55.89% for protein) of JUYP on IL-1β expressions were more significant than that of dexamethasone (DXMS, 61.6% for mRNA, 35.15% for protein) (p
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2020.02.195