Modulation of the Purine Pathway for Riboflavin Production in Flavinogenic Recombinant Strain of the Yeast Candida famata

Riboflavin (vitamin B2) is an indispensable nutrient for humans and animals, since it is the precursor of the essential coenzymes flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD), involved in variety of metabolic reactions. Riboflavin is produced on commercial scale and is used for...

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Bibliographic Details
Published in:Biotechnology journal 2020-07, Vol.15 (7), p.e1900468-n/a
Main Authors: Dmytruk, Kostyantyn V., Ruchala, Justyna, Fedorovych, Daria V., Ostapiv, Roman D., Sibirny, Andriy A.
Format: Article
Language:English
Subjects:
Candida - genetics
Candida - metabolism
Candida famata
Fungal Proteins - genetics
Fungal Proteins - metabolism
Metabolic Engineering - methods
Metabolic Networks and Pathways - genetics
purine biosynthesis
Purines - metabolism
riboflavin
Riboflavin - genetics
Riboflavin - metabolism
yeast
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Summary:Riboflavin (vitamin B2) is an indispensable nutrient for humans and animals, since it is the precursor of the essential coenzymes flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD), involved in variety of metabolic reactions. Riboflavin is produced on commercial scale and is used for feed and food fortification purposes, and in medicine. Until recently, the mutant strains of the flavinogenic yeast Candida famata were used in industry for riboflavin production. Guanosine triphosphate is the immediate precursor of riboflavin synthesis. Therefore, the activation of metabolic flux toward purine nucleotide biosynthesis is a promising approach to improve riboflavin production. The phosphoribosyl pyrophosphate synthetase and phosphoribosyl pyrophosphate amidotransferase are the rate limiting enzymes in purine biosynthesis. Corresponding genes PRS3 and ADE4 from yeast Debaryomyces hansenii are modified to avoid feedback inhibition and cooverexpressed on the background of a previously constructed riboflavin overproducing strain of C. famata. Constructed strain accumulates twofold more riboflavin when compared to the parental strain. Upregulation of purine biosynthesis pathway increases the production of vitamin B2 in the yeast Candida famata.
ISSN:1860-6768
1860-7314
DOI:10.1002/biot.201900468