Adverse effects of in vitro manipulation of spermatozoa

•Sperm selection techniques may damage sperm DNA decreasing implantation in ARTs.•Cryopreservation alters sperm motility, viability and increases DNA damage.•Short- and long-time incubation increase sperm oxidative stress and may damage DNA. Development of in vitro reproduction techniques has not on...

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Bibliographic Details
Published in:Animal reproduction science 2020-09, Vol.220, p.106314-106314, Article 106314
Main Authors: Baldi, Elisabetta, Tamburrino, Lara, Muratori, Monica, Degl’Innocenti, Selene, Marchiani, Sara
Format: Article
Language:English
Subjects:
Assisted reproduction
Sperm cryopreservation
Sperm motility
Sperm selection
Sperm viability
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Summary:•Sperm selection techniques may damage sperm DNA decreasing implantation in ARTs.•Cryopreservation alters sperm motility, viability and increases DNA damage.•Short- and long-time incubation increase sperm oxidative stress and may damage DNA. Development of in vitro reproduction techniques has not only offered some infertile couples the possibility to have a child, it also revolutionized animal reproduction. Although in vitro reproduction techniques for humans or domestic and non-domestic animals have been designed to mimic in vivo conditions, modifications due to environmental effects or in vitro manipulation of gametes and embryos are unavoidable. For male gametes, in vitro manipulations include techniques to select spermatozoa, cryopreservation and other incubation procedures, during which spermatozoa may be exposed to oxidative stress and other insults that may damage their functions and DNA. The aim of this review is to provide an overview of key studies reporting sperm damage during in vitro manipulation, with particular focus on effects on DNA integrity, a fundamental factor for fertilization and transmission of paternal genetic information to offspring.
ISSN:0378-4320
1873-2232
DOI:10.1016/j.anireprosci.2020.106314