Detection of Phytoestrogen Metabolites in Breastfed Infants’ Urine and the Corresponding Breast Milk by Liquid Chromatography–Tandem Mass Spectrometry

To date, there has been limited information on phytoestrogen (PE) exposure and metabolism in breastfed infants. In the present work, 50 sample pairs of Chinese breastfed infants’ urine and the corresponding breast milk were collected. The contents of the relevant PE metabolites in the biosamples wer...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2020-03, Vol.68 (11), p.3485-3494
Main Authors: Min, Jialing, Wang, Zitong, Liang, Chenglin, Li, Wenjie, Shao, Jie, Zhu, Kunrui, Zhou, Lu, Cheng, Jing, Luo, Shihong, Yu, Lehan, Wu, Yudong, Xie, Mingyong, Hu, Xiaojuan
Format: Article
Language:English
Subjects:
Animals
Breast Feeding
Chromatography, Liquid
Female
Humans
Infant
Male
Milk, Human
Phytoestrogens
Pregnancy
Tandem Mass Spectrometry
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Summary:To date, there has been limited information on phytoestrogen (PE) exposure and metabolism in breastfed infants. In the present work, 50 sample pairs of Chinese breastfed infants’ urine and the corresponding breast milk were collected. The contents of the relevant PE metabolites in the biosamples were detected via liquid chromatography–tandem mass spectrometry. The correlations between the PE metabolite contents in breastfed infants’ urine and those in the corresponding breast milk were analyzed. The average concentrations of total PE metabolites in breast milk and urine were 0.27 and 0.23 nmol/mL, respectively. Genistein and enterolactone levels in the infant urine were positively correlated with their concentrations in the corresponding breast milk samples, which implies that urine excretion can be utilized as a noninvasive parameter for precise genistein and enterolactone intake assessment. Additionally, the efficiency of PE urine excretion showed significant differences across infants with different ages, genders, and durations of pregnancy.
ISSN:0021-8561
1520-5118
DOI:10.1021/acs.jafc.9b08107