A UV-B-responsive glycosyltransferase, OsUGT706C2, modulates flavonoid metabolism in rice

• Published in: Science China. Life sciences 2020-07, Vol.63 (7), p.1037-1052
• Main Authors: Zhang, Feng, Guo, Hao, Huang, Jiacheng, Yang, Chenkun, Li, Yufei, Wang, Xuyang, Qu, Lianghuan, Liu, Xianqing, Luo, Jie
• Format: Article
• Language: English
• Subjects: Adaptation, Physiological; Association analysis; Biomedical and Life Sciences; Biosynthetic Pathways; Crop improvement; Enzymatic activity; Flavonoids; Flavonoids - metabolism; Gene Expression Regulation, Plant - radiation effects; Genes, Plant - radiation effects; Genome-Wide Association Study; Genomes; Glycosides; Glycosides - metabolism; Glycosyltransferase; Glycosyltransferases - genetics; Glycosyltransferases - radiation effects; Life Sciences; Lignans - metabolism; Metabolism; Metabolites; Metabolome - radiation effects; Mutation; Oryza - genetics; Plants, Genetically Modified - metabolism; Plants, Genetically Modified - radiation effects; Research Paper; Rice; Transcription
• ISSN: 1674-7305; 1869-1889
• DOI: 10.1007/s11427-019-1604-3

Although natural variations in rice flavonoids exist, and biochemical characterization of a few flavonoid glycosyltransferases has been reported, few studies focused on natural variations in tricin-lignan-glycosides and their underlying genetic basis. In this study, we carried out metabolic profiling of tricin-lignan-glycosides and identified a major quantitative gene annotated as a UDP-dependent glycosyltransferase OsUGT706C2 by metabolite-based genome-wide association analysis. The putative flavonoid glycosyltransferase OsUGT706C2 was characterized as a flavonoid 7-O-glycosyltransferas in vitro and in vivo . Although the in vitro enzyme activity of OsUGT706C2 was similar to that of OsUGT706D1, the expression pattern and induced expression profile of OsUGT706C2 were very different from those of OsUGT706D1 . Besides, OsUGT706C2 was specifically induced by UV-B. Constitutive expression of OsUGT706C2 in rice may modulate phenylpropanoid metabolism at both the transcript and metabolite levels. Furthermore, overexpressing OsUGT706C2 can enhance UV-B tolerance by promoting ROS scavenging in rice. Our findings might make it possible to use the glycosyltransferase OsUGT706C2 for crop improvement with respect to UV-B adaptation and/or flavonoid accumulation, which may contribute to stable yield.