Gastrodin Inhibits H2O2-Induced Ferroptosis through Its Antioxidative Effect in Rat Glioma Cell Line C6

Ferroptosis is a form of necrosis caused by iron-induced accumulation of lipid hydroperoxide, involving several molecular events, and has been implicated in Parkinson’s disease. Gastrodin is a component of Gastrodia elata Blume with strong antioxidant activity. We examined whether gastrodin can prev...

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Published in:Biological & pharmaceutical bulletin 2020/03/01, Vol.43(3), pp.480-487
Main Authors: Jiang, Ting, Chu, Jun, Chen, Hejuntao, Cheng, Hui, Su, Jingjing, Wang, Xuncui, Cao, Yin, Tian, Shasha, Li, Qinglin
Format: Article
Language:English
Subjects:
Antioxidants
Cell death
Cytotoxicity
Deferoxamine
Ferroptosis
gastrodin
Glioma
Glioma cells
Glutathione peroxidase
Heme
Heme oxygenase (decyclizing)
Hydrogen peroxide
L-Lactate dehydrogenase
Lactic acid
Malondialdehyde
Movement disorders
neurodegenerative disease
Neurodegenerative diseases
Oxygenase
Parkinson's disease
Reactive oxygen species
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Summary:Ferroptosis is a form of necrosis caused by iron-induced accumulation of lipid hydroperoxide, involving several molecular events, and has been implicated in Parkinson’s disease. Gastrodin is a component of Gastrodia elata Blume with strong antioxidant activity. We examined whether gastrodin can prevent H2O2-induced cytotoxicity in rat glioma cell line C6. For this purpose, C6 cells were pretreated with gastrodin (1, 5, 25 µM) and then exposed to 100 µM H2O2. Results showed that pretreatment of C6 cells with gastrodin decreased H2O2-induced lactate dehydrogenase (LDH) release and cell death. Moreover, gastrodin decreased intracellular malondialdehyde (MDA) level, whereas increased glutathione peroxidase (GPX) activity and glutathione (GSH) level after H2O2 treatment. In addition, treatment of deferoxamine (DFO), ferrostatin-1, and liproxstatin-1 abolished ferroptosis induced by H2O2 or erastin pretreatment. Treatment with gastrodin attenuated H2O2-induced ferroptosis and decreased lipid reactive oxygen species (ROS) (C11-BODIPY) production in C6 cells. Moreover, gastrodin increased the protein expression of nuclear factor erythroid 2-related factor 2 (Nrf2), GPX4, ferroportin-1 (FPN1), and heme oxygenase-1 (HO-1) in C6 cells treated with H2O2. RSL3, a GPX4 inhibitor, inhibited GPX4 protein level in cells co-treated with gastrodin and 100 µM H2O2. These findings indicate that gastrodin can inhibit H2O2-induced ferroptosis through its antioxidative effect in C6 cells.
ISSN:0918-6158
1347-5215
DOI:10.1248/bpb.b19-00824