Intelligent frequency-shifted optofluidic time-stretch quantitative phase imaging

Optofluidic time-stretch quantitative phase imaging (OTS-QPI) is a powerful tool as it enables high-throughput (>10,000 cell/s) QPI of single live cells. OTS-QPI is based on decoding temporally stretched spectral interferograms that carry the spatial profiles of cells flowing on a microfluidic ch...

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Bibliographic Details
Published in:Optics express 2020-01, Vol.28 (1), p.519-532
Main Authors: Wu, Yunzhao, Zhou, Yuqi, Huang, Chun-Jung, Kobayashi, Hirofumi, Yan, Sheng, Ozeki, Yasuyuki, Wu, Yingli, Sun, Chia-Wei, Yasumoto, Atsushi, Yatomi, Yutaka, Lei, Cheng, Goda, Keisuke
Format: Article
Language:English
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Summary:Optofluidic time-stretch quantitative phase imaging (OTS-QPI) is a powerful tool as it enables high-throughput (>10,000 cell/s) QPI of single live cells. OTS-QPI is based on decoding temporally stretched spectral interferograms that carry the spatial profiles of cells flowing on a microfluidic chip. However, the utility of OTS-QPI is troubled by difficulties in phase retrieval from the high-frequency region of the temporal interferograms, such as phase-unwrapping errors, high instrumentation cost, and large data volume. To overcome these difficulties, we propose and experimentally demonstrate frequency-shifted OTS-QPI by bringing the phase information to the baseband region. Furthermore, to show its boosted utility, we use it to demonstrate image-based classification of leukemia cells with high accuracy over 96% and evaluation of drug-treated leukemia cells via deep learning.
ISSN:1094-4087
1094-4087
DOI:10.1364/OE.380679