Candida biome of severe early childhood caries (S-ECC) and its cariogenic virulence traits

The protected niche of deep-caries lesions is a distinctive ecosystem. We assessed the Candida biome and its cariogenic traits from dentin samples of 50 children with severe-early childhood caries (S-ECC). Asymptomatic, primary molars belonging to International Caries Detection and Assessment-ICDAS...

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Published in:Journal of oral microbiology 2020-01, Vol.12 (1), p.1724484-1724484
Main Authors: Fakhruddin, Kausar Sadia, Perera Samaranayake, Lakshman, Egusa, Hiroshi, Chi Ngo, Hien, Panduwawala, Chamila, Venkatachalam, Thenmozhi, Kumarappan, Allagappan, Pesee, Siripen
Format: Article
Language:English
Subjects:
acidogenicity
biofilm
calcium-release
Candida species
dentin caries
haemolysin
hydrolases
Original
phopholipase
protease
severe early childhood caries (S-ECC)
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Summary:The protected niche of deep-caries lesions is a distinctive ecosystem. We assessed the Candida biome and its cariogenic traits from dentin samples of 50 children with severe-early childhood caries (S-ECC). Asymptomatic, primary molars belonging to International Caries Detection and Assessment-ICDAS caries-code 5 and 6 were analyzed, and C. albicans (10-isolates), C. tropicalis (10), C. krusei (10), and C. glabrata (5) isolated from the lesions were then evaluated for their biofilm formation, acidogenicity, and the production of secreted hydrolases: hemolysins, phospholipase, proteinase and DNase. Candida were isolated from 14/43 ICDAS-5 lesions (32.5%) and 44/57 ICDAS-6 lesions (77.2%). Compared to, ICDAS-5, a significantly higher frequency of multi-species infestation was observed in ICDAS-6 lesions (p=0.001). All four candidal species (above) showed prolific biofilm growth, and an equal potency for tooth demineralization. A significant interspecies difference in the mean phospholipase, as well as proteinase activity was noted (p 
ISSN:2000-2297
2000-2297
DOI:10.1080/20002297.2020.1724484