TGF-β3/Smad3 Contributes to Isoflurane Postconditioning Against Cerebral Ischemia–Reperfusion Injury by Upregulating MEF2C

• Published in: Cellular and molecular neurobiology 2020-11, Vol.40 (8), p.1353-1365
• Main Authors: Yang, Yuqi, Chen, Long, Si, Junqiang, Ma, Ketao, Yin, Jiangwen, Li, Yan, Yang, Chengwei, Wang, Sheng
• Format: Article
• Language: English
• Subjects: Apoptosis; Biomedical and Life Sciences; Biomedicine; Cell Biology; Cerebral blood flow; Ischemia; Isoflurane; Myocytes; Neurobiology; Neuroprotection; Neurosciences; Original Research; Reperfusion; Signal transduction; Smad3 protein; Transforming growth factor-b1
• ISSN: 0272-4340; 1573-6830
• DOI: 10.1007/s10571-020-00822-5

Isoflurane postconditioning alleviates cerebral ischemic–reperfusion injury (CIRI), but the underlying mechanism has not been fully clarified. We previously demonstrated that the transforming growth factor beta-1 (TGF-β1)/Smads signaling pathway is involved in the neuroprotective effect of isoflurane postconditioning. TGF-β3 has a highly homologous sequence relative to that of TGF-β1. In this study, we explored the roles of the TGF-β3/Smad3 signaling pathway and myocyte enhancer factor 2C (MEF2C) in neuroprotection induced by isoflurane postconditioning. A CIRI rat model was established by middle cerebral artery occlusion for 1.5 h, followed by 24 h of reperfusion. Isoflurane postconditioning led to lower infarct volumes and neurologic deficit scores, more surviving neurons, and less damaged and apoptotic neurons as compared with those of CIRI rats. Moreover, isoflurane postconditioning upregulated the expressions of TGF-β3, p-Smad3, and MEF2C. However, the neuroprotective effect was reversed by pirfenidone, a TGF-β3/Smad3 signaling pathway inhibitor. Also, pirfenidone treatment downregulated the expression of MEF2C. These results indicate that the TGF-β3/Smad3 signaling pathway contributes to the neuroprotection of isoflurane postconditioning after CIRI and is possibly related to MEF2C.