Evaluation of citrus flavonoids against Aspergillus parasiticus in maize: Aflatoxins reduction and ultrastructure alterations

•Flavonoids have been shown to inhibit aflatoxins by Aspergillus parasiticus in maize.•A ternary mixture of flavonoids reduced more than 85% the accumulation of aflatoxins.•Flavonoids caused severe morphological alterations in A. parasiticus ultrastructure.•Flavonoids could be used as antimycotoxin...

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Bibliographic Details
Published in:Food chemistry 2020-07, Vol.318, p.126414-126414, Article 126414
Main Authors: Pok, Paula Sol, García Londoño, Víctor Alonso, Vicente, Sebastián, Romero, Stella Maris, Pacín, Ana, Tolaba, Marcela, Alzamora, Stella Maris, Resnik, Silvia Liliana
Format: Article
Language:English
Subjects:
Aflatoxins
Aspergillus parasiticus
Maize
Naringin
Neohesperidin
Quercetin
Ultrastructure
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Summary:•Flavonoids have been shown to inhibit aflatoxins by Aspergillus parasiticus in maize.•A ternary mixture of flavonoids reduced more than 85% the accumulation of aflatoxins.•Flavonoids caused severe morphological alterations in A. parasiticus ultrastructure.•Flavonoids could be used as antimycotoxin agents, replacing synthetic additives. In this study, the effects of citrus flavonoids naringin (NAR), neohesperidin (NEO) and quercetin (QUER) on aflatoxins accumulation by a selected Aspergillus parasiticus strain in maize at 0.95 aw were studied by response surface methodology using a Box-Behnken design. Multiple response optimization was applied to simultaneously minimize the contamination with aflatoxins (AFs) B1, G1, B2 and G2. The application of the optimal mixture in maize at 0.95 aw (0.39 mM NAR, 0.24 mM NEO and 0.40 mM QUER) reduced from 85% to 100% AFs accumulation. The same mixture at 0.98 aw, led to a reduction in AFs accumulation that ranged from 93% to 98%. Ultrastructure alterations of cellular membranes and walls in A. parasiticus, evidenced by transmission electron microscopy images, were severe and depended on the type of flavonoid and their combination. Flavonoid mixtures may provide an environmentally friendly alternative for decreasing AFs accumulation in stored maize, replacing synthetic compounds.
ISSN:0308-8146
1873-7072
DOI:10.1016/j.foodchem.2020.126414