rs1016860 of BCL2 3'UTR associates with hsa-miR-629-5p binding potential in breast cancer and gastric cancer in Isfahan population

• Published in: Gene 2020-05, Vol.738, p.144457-144457
• Main Authors: Hashemi Doulabi, Mahsa Sadat, Ghaedi, Kamran, Ranji, Najmeh, Khazaei Koohpar, Zeinab
• Format: Article
• Language: English
• Subjects: 3' Untranslated Regions - genetics; 3' Untranslated Regions - physiology; Adult; Alleles; Apoptosis - genetics; Breast Neoplasms - genetics; Carcinogenesis - genetics; Case-Control Studies; Female; Genetic Predisposition to Disease; Genotype; Humans; Iran - epidemiology; MicroRNAs - genetics; MicroRNAs - metabolism; Middle Aged; Neoplasm Staging; Polymorphism, Single Nucleotide - genetics; Proto-Oncogene Proteins c-bcl-2 - genetics; Proto-Oncogene Proteins c-bcl-2 - metabolism; Receptors, Progesterone - genetics; Stomach Neoplasms - genetics
• ISSN: 1879-0038
• DOI: 10.1016/j.gene.2020.144457

Breast cancer is caused by the interaction of inherited and environmental risk factors. Also, gastric cancer is the second fatal carcinoma. B-cell leukemia/lymphoma 2 gene family plays a crucial role in carcinogenesis by inhibiting the apoptosis process. In this study, 129 patients with breast cancer and 132 controls as well as 136 patients with gastric cancer and 50 controls were enrolled. We used Real time PCR to determine the genotype of the samples. Finally, we analyzed the diagram based on high temperature melting curve diagram using the MICPCR software, followed by bioinformatics prediction of rs1016860 functions. rs1016860 of BCL2 gene with CC, CT, and TT genotypes were observed in this region. The association of Estrogen receptor and Progesterone receptor, cancer stage and grade of cancer in the patients with genotypes was significant in breast cancer. The association of the status of primary tumor in the patients with genotypes is significant in gastric cancer (Chi-Square p