A Sensitive Electrochemical Ascorbic Acid Sensor Using Glassy Carbon Electrode Modified by Molybdenite with Electrodeposited Methylene Blue

• Published in: Applied biochemistry and biotechnology 2020-08, Vol.191 (4), p.1533-1544
• Main Authors: Zhang, Yan, Wang, Yue, Hasebe, Yasushi, Zhang, Zhiqiang, Tao, Dongping
• Format: Article
• Language: English
• Subjects: Ascorbic acid; Ascorbic Acid - chemistry; Biochemistry; Biotechnology; Carbon - chemistry; Catalysis; Chemistry; Chemistry and Materials Science; Electrical measurement; Electrochemical Techniques - methods; Electrochemistry; Electrodes; Electrolytes; Fabrication; Glassy carbon; High temperature; Hydrogen-Ion Concentration; Limit of Detection; Linear Models; Methylene blue; Methylene Blue - chemistry; Molybdenite; Molybdenum - chemistry; Morphology; Reproducibility; Reproducibility of Results; Response time; Selectivity; Sensitivity and Specificity; Sensors
• ISSN: 0273-2289; 1559-0291
• DOI: 10.1007/s12010-020-03255-4

A non-enzymatic amperometric sensor using natural molybdenite (MLN) electrodeposited with methylene blue (MB) has been fabricated and characterized and its analytical performances were investigated for the determination of ascorbic acid (AA). The surface morphology of the electrode modified by electrodeposited MB was studied by use of the Advanced Mineral Identification and Characterization System (AMICS) and laser confocal high-temperature scanning microscope (LCSM). The poly(MB) and MLN immobilized sensor showed good stability, reproducibility, sensitivity, and selectivity. It exhibited a linear performance range from 3 to 1000 μM, with a lower detection limit of 0.083 μM (signal/noise = 3) and short response time (