Preliminary investigation on a new natural based poly(gamma‐glutamic acid)/Chitosan bioink

The study aims to investigate a novel bioink made from Chitosan (Cs)/ poly(gamma‐glutamic acid) (Gamma‐PGA) hydrogel that takes advantage of the two biodegradable and biocompatible polymers meeting most of the requirements for biomedical applications. The bioink could be an alternative to other mate...

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Published in:Journal of biomedical materials research. Part B, Applied biomaterials Applied biomaterials, 2020-10, Vol.108 (7), p.2718-2732
Main Authors: Pisani, Silvia, Dorati, Rossella, Scocozza, Franca, Mariotti, Camilla, Chiesa, Enrica, Bruni, Giovanna, Genta, Ida, Auricchio, Ferdinando, Conti, Michele, Conti, Bice
Format: Article
Language:English
Subjects:
3D‐bioprinting
Alginates
Alginic acid
Biocompatibility
Biodegradability
Biodegradation
Bioengineering
Biomedical materials
CAD
Cell culture
Cell viability
Chitosan
Complex formation
Computer aided design
Extrusion
Fibroblasts
Gelatin
Gelation
Glutamic acid
hydrogel
Hydrogels
interpolyelectrolyte complex
Materials research
Materials science
poly(gamma‐glutamic acid)
Polymers
Rheological properties
Rheology
Shear stress
Stability analysis
Three dimensional models
Three dimensional printing
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container_title Journal of biomedical materials research. Part B, Applied biomaterials
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creator Pisani, Silvia
Dorati, Rossella
Scocozza, Franca
Mariotti, Camilla
Chiesa, Enrica
Bruni, Giovanna
Genta, Ida
Auricchio, Ferdinando
Conti, Michele
Conti, Bice
description The study aims to investigate a novel bioink made from Chitosan (Cs)/ poly(gamma‐glutamic acid) (Gamma‐PGA) hydrogel that takes advantage of the two biodegradable and biocompatible polymers meeting most of the requirements for biomedical applications. The bioink could be an alternative to other materials commonly used in 3D‐bioprinting such as gelatin or alginate. Cs/ Gamma‐PGA hydrogel was prepared by double extrusion of Gamma‐PGA and Cs solutions, where 2 × 105 human adult fibroblasts per ml Cs solution had been loaded, through Cellink 3D‐Bioprinter at 37°C. A computer aided design model was used to get 3D‐bioprinting of a four layers grid hydrogel construct with 70% infill. Hydrogel characterization involved rheology, FTIR analysis, stability study (mass loss [ML], fluid uptake [FU]), and cell retaining ability into hydrogel. 3D‐bioprinted hydrogel gelation time resulted to be
doi_str_mv 10.1002/jbm.b.34602
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The bioink could be an alternative to other materials commonly used in 3D‐bioprinting such as gelatin or alginate. Cs/ Gamma‐PGA hydrogel was prepared by double extrusion of Gamma‐PGA and Cs solutions, where 2 × 105 human adult fibroblasts per ml Cs solution had been loaded, through Cellink 3D‐Bioprinter at 37°C. A computer aided design model was used to get 3D‐bioprinting of a four layers grid hydrogel construct with 70% infill. Hydrogel characterization involved rheology, FTIR analysis, stability study (mass loss [ML], fluid uptake [FU]), and cell retaining ability into hydrogel. 3D‐bioprinted hydrogel gelation time resulted to be &lt;60 s, hydrogel structure was maintained up to 36.79 Pa shear stress, FTIR analysis demonstrated Gamma‐PGA/Cs interpolyelectrolyte complex formation. The 3D‐bioprinted hydrogel was stable for 35 days (35% ML) in cell culture medium, with increasing FU. 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subjects 3D‐bioprinting
Alginates
Alginic acid
Biocompatibility
Biodegradability
Biodegradation
Bioengineering
Biomedical materials
CAD
Cell culture
Cell viability
Chitosan
Complex formation
Computer aided design
Extrusion
Fibroblasts
Gelatin
Gelation
Glutamic acid
hydrogel
Hydrogels
interpolyelectrolyte complex
Materials research
Materials science
poly(gamma‐glutamic acid)
Polymers
Rheological properties
Rheology
Shear stress
Stability analysis
Three dimensional models
Three dimensional printing
title Preliminary investigation on a new natural based poly(gamma‐glutamic acid)/Chitosan bioink
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