Investigation on revascularization time and initial damage after transplantation of fresh and cryopreserved ovarian tissue in domestic cats

The present study evaluated revascularization time of fresh and cryopreserved cat ovarian tissue after transplantation to subcutaneous tissue. Ovaries of five cats were used and eight pieces of ovarian tissue were taken from each pair of ovaries. Immediately after removal, three pieces were transpla...

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Bibliographic Details
Published in:Cell and tissue banking 2020-06, Vol.21 (2), p.303-312
Main Authors: da Costa, Marcella Motta, Gonçalves, Liudimila Passos, Lemos, Marcelle Santos, Marangon, Aline Ramos Marques, Lucci, Carolina Madeira
Format: Article
Language:English
Subjects:
Animals
Biomedical and Life Sciences
Biomedicine
Blood vessels
Cats
Cell Biology
Cryopreservation
Cryopreservation - veterinary
Doppler effect
Female
Follicles
Full Length Paper
Immunohistochemistry
Life Sciences
Ovarian Follicle - transplantation
Ovaries
Ovary - blood supply
Ovary - transplantation
Thawing
Time Factors
Transplant Surgery
Transplantation
Ultrasonic imaging
Ultrasonography, Doppler
Ultrasound
Vascularization
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Summary:The present study evaluated revascularization time of fresh and cryopreserved cat ovarian tissue after transplantation to subcutaneous tissue. Ovaries of five cats were used and eight pieces of ovarian tissue were taken from each pair of ovaries. Immediately after removal, three pieces were transplanted and one fixed for fresh control. The remaining four pieces were cryopreserved and, after thawing, one was fixed for cryopreservation control and three were transplanted. Grafts were recovered on days 2 (D2), 4 (D4) and 6 (D6) post-transplantation. Blood vessels were identified by immunohistochemistry and doppler ultrasound. Immunohistochemistry showed that the percentages of total tissue area occupied by blood vessels were similar ( P  > 0.05) in fresh and cryopreserved tissues. In both cases, blood vessel area was significantly higher ( P  
ISSN:1389-9333
1573-6814
DOI:10.1007/s10561-020-09823-6