PdIrBP mesoporous nanospheres combined with superconductive carbon black for the electrochemical determination and collection of circulating tumor cells

An integrated electrochemical immunoassay is described for the determination of circulating tumor cells (CTCs). For the first time, Ketjen black (KB), which is a superconductive carbon material, was incorporated with Au nanoparticles (AuNPs) and used to modify the surface of gold electrodes. A cockt...

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Bibliographic Details
Published in:Mikrochimica acta (1966) 2020-04, Vol.187 (4), p.216-216, Article 216
Main Authors: Peng, Yang, Peng, Yuhang, Tang, Sitian, Shen, Huawei, Sheng, Shangchun, Wang, Yonghong, Wang, Teng, Cai, Juan, Xie, Guoming, Feng, Wenli
Format: Article
Language:English
Subjects:
Alloys
Analytical Chemistry
Antibodies
Carbon
Carbon black
Cell adhesion
Cell adhesion & migration
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Electric properties
Electrodes
Glycine
Gold
Immunoassay
Iridium
Metastasis
Microengineering
Nanochemistry
Nanoparticles
Nanospheres
Nanotechnology
Original Paper
Palladium
Superconductors
Tumors
Viral antibodies
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Summary:An integrated electrochemical immunoassay is described for the determination of circulating tumor cells (CTCs). For the first time, Ketjen black (KB), which is a superconductive carbon material, was incorporated with Au nanoparticles (AuNPs) and used to modify the surface of gold electrodes. A cocktail of anti-epithelial cell adhesion molecules (EpCAM) and anti-vimentin antibodies was chosen to capture the CTCs. Palladium-iridium-boron-phosphorus alloy-modified mesoporous nanospheres (PdIrBPMNS) served as a catalytic tag to amplify the current signal. Glycine-HCl (Gly-HCl) was used as an antibody eluent to release and collect the captured CTCs from the electrodes for further clinical research without compromising cell viability. The response of the method increased linearly from 10 to 1 × 10 6 cells mL −1 CTCs, while the detection limit was calculated to be as low as 2 cells mL −1 . This method was successfully used to determine CTCs in spiked blood samples and demonstrated good recovery. Graphical abstract Ketjen black/AuNPs was incorporated in the electrochemical platform to enhance the electron transfer ability of the electrode surface. PdIrBP mesoporous nanospheres were used to amplify DPV signal in this assay. The introduction of Gly-HCl realized nondestructive recovery of circulating tumor cells.
ISSN:0026-3672
1436-5073
DOI:10.1007/s00604-020-4213-z