A Method to Analyze Urinary Extracellular Vesicles

Extracellular vesicles (EVs) play an important role in cell-to-cell communication by carrying molecular messages that reflect physiological and pathological conditions of the parent cells. EVs have been identified in all body fluids; and among them, urine stands out as a sample that is easy and inex...

Full description

Saved in:
Bibliographic Details
Published in:Analytical Sciences 2020/07/10, Vol.36(7), pp.791-798
Main Authors: PAISRISARN, Piyawan, YASUI, Takao, BABA, Yoshinobu
Format: Article
Language:English
Subjects:
Analytical Chemistry
analytical method
Analytical methods
Biomarkers
Biomarkers - urine
Body fluids
cancer
Cell interactions
characterization
Chemistry
Extracellular vesicles
Extracellular Vesicles - chemistry
Humans
isolation
Qualitative analysis
Quantitative analysis
Reviews
Urinary extracellular vesicles
Vesicles
Workflow
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Extracellular vesicles (EVs) play an important role in cell-to-cell communication by carrying molecular messages that reflect physiological and pathological conditions of the parent cells. EVs have been identified in all body fluids; and among them, urine stands out as a sample that is easy and inexpensive to obtain and can be collected over time to monitor changes. Various protocols have been established to study urinary extracellular vesicles (UEVs) and they have shown great potential as a biomarker source for clinical applications, not only for urological, but also non-urological diseases. Due to the high variability and low reproducibility of pre-analytical and analytical methods for UEVs, establishing a standardized protocol remains a challenge in the field of diagnosis. Here, we review UEV studies and present the techniques that are most commonly used, those that have been applied as new developments, and those that have the most potential for future applications. The workflow procedures from the sampling step to the qualitative and quantitative analysis steps are summarized along with advantages and disadvantages of the methodologies, in order to give consideration for choosing the most promising and suitable method to analyze human UEVs.
ISSN:0910-6340
1348-2246
DOI:10.2116/analsci.20R001