Dynamic changes of the bacterial communities in roast chicken stored under normal and modified atmosphere packaging

• Published in: Journal of food science 2020-04, Vol.85 (4), p.1231-1239
• Main Authors: Huang, Jichao, Guo, Yuchen, Hou, Qin, Huang, Ming, Zhou, Xinghu
• Format: Article
• Language: English
• Subjects: 16S rDNA; Abundance; Atmosphere; Bacteria; bacterial community; Carbon dioxide; Chickens; Flora; Food packaging; Food safety; Low temperature; Meat; Microorganisms; modified atmosphere packaging (MAP); normal packaging; Operators; Organisms; Packaging; Poultry; Pseudomonas; Relative abundance; roast chicken; rRNA 16S; Spoilage; Storage; Storage temperature
• ISSN: 0022-1147; 1750-3841
• DOI: 10.1111/1750-3841.15038

This study systematically investigated the dynamic changes in bacterial communities in roast chicken in normal and modified atmosphere packaging (MAP). The samples were stored under normal atmosphere and 40%/60% CO2/N2 MAP conditions for 28 days at 4 °C. Changes in the number and type of microorganisms in roast chicken during storage were defined via cultural and 16S rDNA sequencing techniques. More Bacteroides, Chryseobacterium, Lactobacillus, and Acinetobacter than other bacteria were initially found in roast chicken. With normal packaging, Pseudomonas rapidly multiplied and became the main spoilage organism in roast chicken after 7 days, with a relative abundance of >90% of the entire bacterial flora. With MAP, due to the high salt content, Halomonas became the main spoilage organism in roast chicken by the middle of the storage period (14 days). Between days 21 and 28 of storage, Pseudomonas gradually became the main spoilage organism in roast chicken, but its relative abundance was much lower in MAP than in normal packaging, followed by Lachnospiraceae (NK4A136 group) and Altererythrobacter. Our research shows that the microbes in roast chicken mainly originated from the processing environment and operators. The combination of MAP with a low storage temperature could effectively improve the quality and safety of roast chicken meat. Practical Applications This research showed the dynamic changes in the bacterial community of roast chicken stored under normal and modified atmosphere packaging (MAP). Microorganisms in roast chicken are mainly obtained from the processing environment and operators. Combining MAP with storage at low temperatures can effectively improve the quality and safety of roast chicken.