Oxidations in white grape (Vitis vinifera L.) skins: Comparison between ripening process and photooxidative sunburn symptoms

• Published in: Plant physiology and biochemistry 2020-05, Vol.150, p.270-278
• Main Authors: Rustioni, Laura, Fracassetti, Daniela, Prinsi, Bhakti, Geuna, Filippo, Ancelotti, Alessandro, Fauda, Valerio, Tirelli, Antonio, Espen, Luca, Failla, Osvaldo
• Format: Article
• Language: English
• Subjects: Chemical composition; Enzymatic activities; Fruit ripening; Grape (Vitis vinifera L.); Oxidative stress; Reflectance spectroscopy; Sunburn
• ISSN: 0981-9428; 1873-2690
• DOI: 10.1016/j.plaphy.2020.03.003

Oxidations in grape berries are gaining major interest as they affect grape characteristics and quality. Considering berries, Reactive Oxygen Species are involved in the responses to both ripening process and stresses, including photooxidative sunburn. Redox metabolism involves a multitude of chemical and enzymatic reactions. In this study, four white grape cultivars were examined for natural ripening and photooxidative sunburn effects (obtained in artificial conditions) on berry pigmentation, chemical composition and enzymatic activity. The measured parameters included reflectance spectra, pigmentation (including berry browning), content of photosynthetic pigments, organic acid profiles, antioxidant activity, concentrations of antioxidants (total phenolics, ascorbic acid and reduced glutathione), enzymatic activities (guaiacol peroxidases, ascorbate peroxidase and catalase). The effects of the treatment (natural ripening and artificial photooxidative sunburn) on each considered parameter are described in the paper. Photooxidative sunburn strongly affected the contents of antioxidants and chlorophylls, increased the browning index and modulated the enzymatic activities investigated. Samples clearly clustered depending on the oxidation status. Furthermore, the PCA highlighted the similarities and differences in the responses to oxidative stress during ripening and photooxidative sunburn. PCA produced five functions with eigenvalues higher than 1, representing 87.03% of the total variability. In particular, the scores of the function 1 discriminated the samples based on the oxidation status, while the function 2 separated the samples based on the sampling date, representing the physiological responses characteristic of ripening. Our work sheds light on this topic, and will allow a more conscious vineyard management, thus supporting the agricultural adaptation to climate changes. •Ripening and artificial sunburn effects were studied in 4 white grape cultivars.•Chemical and enzymatic redox metabolisms were investigated.•PCA produced five functions with eigenvalues higher than 1.•Function 1 is representative of sample oxidation and function 2 of natural ripening.