Enzymatic preparation of chitooligosaccharides and their anti-obesity application

• Published in: Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2020-07, Vol.84 (7), p.1460-1466
• Main Authors: Zhou, Yu, Li, Shangyong, Li, Dandan, Wang, Shuo, Zhao, Wandong, Lv, Zhiyuan, Li, Xiao, Li, Haoyan, Han, Yantao
• Format: Article
• Language: English
• Subjects: Animals; anti-obesity; Anti-Obesity Agents - administration & dosage; Anti-Obesity Agents - chemical synthesis; Anti-Obesity Agents - pharmacology; Chitin - administration & dosage; Chitin - analogs & derivatives; Chitin - chemical synthesis; Chitin - pharmacology; chitooligosaccharides; Chitosan; Chitosan - chemistry; Cholesterol, HDL - blood; Cholesterol, LDL - blood; Diet, High-Fat - adverse effects; Disease Models, Animal; Fatty Acids, Nonesterified - blood; Glycoside Hydrolases - chemistry; Hydrogen-Ion Concentration; Hydrolysis; Male; Mice; Obesity - blood; Obesity - drug therapy; Obesity - etiology; Pseudoalteromonas - enzymology; Temperature; Triglycerides - blood
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1080/09168451.2020.1744110

Chitooligosaccharides (COS) are derived from chitosan, which can be used as nutraceuticals and functional foods. Because of their various biological activities, COS are widely used in the food, medicine, agriculture, and other fields. COS were prepared by chitosanase  from Pseudoalteromonas sp. SY39 and their anti-obesity activity was researched in mice in this study. The effects of hydrolysis time, temperature, the ratio of enzyme to chitosan, and pH on the productivity of COS were discussed. Preparation process of COS was established in a 5-L fermenter. COS were characterized and their anti-obesity activity was studied in animal experiments. The results showed that COS could effectively reduce serum lipid levels and obesity in mice, and have a good anti-obesity activity. The preparation technology and remarkable anti-obesity activity of COS further expand their applications in the food and pharmaceutical industries. Chitooligosaccharides (COS) were effectively prepared by using chitosanase under the optimum conditions, which had excellent anti-obesity activity in mice.