The influence of MoO3-NPs on agro-morphological criteria, genomic stability of DNA, biochemical assay, and production of common dry bean (Phaseolus vulgaris L.)

• Published in: Plant physiology and biochemistry 2020-06, Vol.151, p.77-87
• Main Authors: Osman, Samira A., Salama, Dina M., Abd El-Aziz, M.E., Shaaban, Essam A., Abd Elwahed, Mohamed S.
• Format: Article
• Language: English
• Subjects: Agro-morphological criteria; Genotoxicity; ISSR-PCR; Molybdenum oxide nanoparticles; Phaseolus vulgaris; SDS-PAGE; Yield
• ISSN: 0981-9428; 1873-2690
• DOI: 10.1016/j.plaphy.2020.03.009

Molybdenum is considered one of the most important micronutrients applied as a foliar fertilizer for common dry bean. In this study, molybdenum oxide nanoparticles (MoO3-NPs) were applied in different concentrations (0, 10, 20, 30 and 40 ppm) over two sequent seasons, 2018 and 2019, to investigate their effect on the plant morphological criteria, yield, and the genomic stability of DNA. The results showed that the application of 40 ppm MoO3-NPs as a foliar fertilizer showed preferable values of plant morphological criteria, such as the number of leaves and branches per plant, as well as the fresh and dry weight with regard to the common bean plant. In addition, the seed yield increased by 82.4% and 84.1% with 40 ppm, while the shoot residue increased by 32.2% and 32.1% with 20 ppm of MoO3-NPs during two seasons, 2018 and 2019, respectively. Furthermore, the common bean treated with 20 and 40 ppm MoO3-NPs had positive unique bands with ISSR primer 848 at 1400 bp (Rf 0.519) and with primer ISSR2M at 200 bp (Rf 0.729), respectively. In addition, SDS-PAGE reveald some proteins in seedlings which were absent in the flowering stage at 154, 102, 64, 37 and 34 KDa, which may be due to differences in plant proteins required for metabolic processes in each stage. In conclusion, the application of 40 ppm MoO3-NPs was more effective on the productivity of the common bean plants. [Display omitted] •MoO3-NPs improve the plant criteria of common bean.•MoO3-NPs increase the yield of common bean seeds by 82%.•MoO3-NPs can be endorsed for production of common bean.•Genotoxic effect of MoO3-NPs on genomic DNA of common bean.•MoO3-NPs up to 40pp effect on GTS of common bean.