Effects of phenolic acids on free radical scavenging and heavy metal bioavailability in kandelia obovata under cadmium and zinc stress

• Published in: Chemosphere (Oxford) 2020-06, Vol.249, p.126341-126341, Article 126341
• Main Authors: Chen, Shan, Lin, Ruiyu, Lu, Haoliang, Wang, Qiang, Yang, Jinjin, Liu, Jingchun, Yan, Chongling
• Format: Article
• Language: English
• Subjects: Antioxidants - metabolism; Bioavailability; Biological Availability; Cadmium; Cadmium - chemistry; Cadmium - metabolism; Caffeic Acids; Coumaric Acids; Enzymatic system; Hydroxybenzoates - chemistry; Kandelia obovata; Metals, Heavy - chemistry; Metals, Heavy - metabolism; Phenolic acid; Rhizophoraceae - drug effects; Rhizophoraceae - physiology; Wetlands; Zinc; Zinc - chemistry; Zinc - metabolism
• ISSN: 0045-6535; 1879-1298
• DOI: 10.1016/j.chemosphere.2020.126341

Current mechanism studies in plant heavy metal tolerance do not consider the effects of different phenolic acids on the bioavailability of heavy metals and the comparison with antioxidant enzyme system in the hydroxyl radical scavenging capacity. In present study, by a set of pot culture experiments with adding cadmium (Cd) and zinc (Zn) to the sediments, the effects of different phenolic acids on the toxicity of Cd and Zn in Kandelia obovata and the dominant role in scavenging hydroxyl radicals were evaluated. The results showed that 100 mg kg−1 Zn treatment promoted the growth of plant under high concentrations of Cd and Zn stress. Under the stress of Cd and Zn, the phenolic acids were mainly metabolized by phenylpropanoid and flavonoid pathways, supplemented by shikimate and monolignol pathways in K. obovata. Eleven phenolic acids with different abilities of scavenging free radicals were detected in the plant, including pyrogallic acid (Gal), coumaric acid (Cou), protocatechuic acid (Pro), chlorogenic acid (Chl), 4-hydroxy benzoic acid (Hyd), caffeic acid (Caf), vanillic acid (Van), ferulic acid (Fer), benzoic acid (Ben), and salicylic acid (Sal). By adding phenolic acids to the sediments, chlorogenic acid (Chl), pyrogallic acid (Gal), cinnamic acid (Cin), and coumaric acid (Cou) behave as more reactive in changing Cd or Zn into residual fractions than the others, and chlorogenic acid (Chl), pyrogallic acid (Gal), ferulic acid (Fer) and caffeic acid (Caf) have higher ability of scavenging hydroxyl radicals than the others. In summary, K. obovata tends to synthesize phenolic acids with strong scavenging ability of free radicals and changing the bioavailability of Cd and Zn under high concentration of Cd and Zn stress. Phenolic acids played a crucial role in the mitigative effect of heavy metal stress via scavenging free radicals and involving in the process of Cd and Zn uptake and tolerance. The results will provide important theoretical basis and method guidance for mangrove wetland conservation. [Display omitted] •Kandelia obovata phenolic acid metabolism is involved in alleviating the toxic process of cadmium and zinc.•Phenolic acids can remove hydroxyl radicals better than antioxidant enzymes.•Four phenolic metabolism pathways participate in alleviating the toxic of Cd and Zn.•Different phenolic acids have different ways to alleviate the oxidative damage caused by Cd and Zn stress.•Kandelia obovata is selective in the synthesis and secretion of pheno