Biogenic synthesis and antimicrobial activity of silica-coated silver nanoparticles for esthetic dental applications

this study aimed to synthesize AgNPs from green tea (GT) extract, forming GT-AgNPs, and to coat their surfaces with silica, resulting in light-colored Ag@SiO2 nanoparticles. particles were characterized and tested for minimal inhibitory concentration (MIC), biofilm formation against Streptococcus mu...

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Bibliographic Details
Published in:Journal of dentistry 2020-05, Vol.96, p.103327-103327, Article 103327
Main Authors: Rodrigues, Marcela Charantola, Rolim, Wallace Rosado, Viana, Marina Mariante, Souza, Thaís Rodrigues, Gonçalves, Flavia, Tanaka, Caio Junji, Bueno-Silva, Bruno, Seabra, Amedea Barozzi
Format: Article
Language:English
Subjects:
Ag@SiO2 nanoparticles
Antibacterial activity
Antiinfectives and antibacterials
Antimicrobial activity
Antimicrobial agents
Aqueous solutions
Bacteria
Biocompatibility
Biofilms
Biogenic synthesis
Biomaterials
Biomedical materials
Brownian motion
Cytotoxicity
Dental caries
Dental materials
Dental pulp
Dentistry
Ethanol
Fibroblasts
Green chemistry
Green tea
Incorporation
Infrared analysis
Infrared tracking
Light scattering
Minimum inhibitory concentration
Nanoparticles
Photon correlation spectroscopy
Polydispersity
Resins
Silica
Silicon dioxide
Silver
Silver base alloys
Silver nanoparticles
Sodium
Software
Spectrum analysis
Surface area
Tea
Thermogravimetric analysis
Toxicity
Transmission electron microscopy
X-ray diffraction
X-ray spectroscopy
Zeta potential
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Description
Summary:this study aimed to synthesize AgNPs from green tea (GT) extract, forming GT-AgNPs, and to coat their surfaces with silica, resulting in light-colored Ag@SiO2 nanoparticles. particles were characterized and tested for minimal inhibitory concentration (MIC), biofilm formation against Streptococcus mutans and cytotoxicity evaluation on dental pulp fibroblasts. X-ray diffraction (XRD) confirmed the formation of pure AgNPs, whereas energy dispersive X-ray spectroscopy (EDS) mapped their elemental atoms. Dynamic light scattering (DLS) demonstrated formation of particles at nanoscale, with moderate polydispersity and negative zeta potential, in agreement with nanoparticle tracking analysis (NTA) size measurements. Fourier-transformed infrared (FTIR) spectroscopy confirmed the successful condensation of silica, which significantly increased surface area by 50%, as assayed by surface area analysis (BET). Thermogravimetric analysis showed a 18%-mass of silica on the surface of Ag@SiO2NPs. Transmission electron microscopy (TEM) displayed the spherical shape of nanoparticles and average size of 11 nm for GT-AgNPs and Ag@SiO2NPs. Ag@SiO2NPs demonstrated potent antimicrobial action against S. mutans, with MIC determined as 600 μg/mL, and inhibition of approximately 44% (p 
ISSN:0300-5712
1879-176X
DOI:10.1016/j.jdent.2020.103327