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Prevention of denervated muscle atrophy with accelerated nerve‐regeneration by babysitter procedure in rat facial nerve paralysis model
Purpose The “babysitter” procedure is a reconstruction technique for facial nerve complete paralysis and uses the movement source from the healthy facial nerve with a cross‐nerve graft. First, an end‐to‐side neurorrhaphy is performed between the affected facial nerve trunk and hypoglossal nerve for...
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| Published in: | Microsurgery 2021-01, Vol.41 (1), p.61-69 |
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| container_start_page | 61 |
| container_title | Microsurgery |
| container_volume | 41 |
| creator | Hashimoto, Kazuki Matsumine, Hajime Osaki, Hironobu Ueta, Yoshifumi Kamei, Wataru Shimizu, Mari Fujii, Kaori Niimi, Yosuke Miyata, Mariko Sakurai, Hiroyuki |
| description | Purpose
The “babysitter” procedure is a reconstruction technique for facial nerve complete paralysis and uses the movement source from the healthy facial nerve with a cross‐nerve graft. First, an end‐to‐side neurorrhaphy is performed between the affected facial nerve trunk and hypoglossal nerve for continuously delivering stimuli to the mimetic muscles for preventing the atrophy of mimetic muscles. Despite favorable clinical results, histological and physiological mechanisms remain unknown. This study attempted to establish a model for the “babysitter” procedure and find its efficacy in rats with facial nerve complete paralysis.
Materials and Methods
A total of 16 Lewis rats were used and divided into 2 groups; cross nerve graft (n = 8) and babysitter groups (n = 8). The facial nerve trunk was transected in both groups. Babysitter group underwent a two‐stage procedure. Cross nerve graft group underwent only the transfer of nerve graft from the healthy side to affected side. The animals were assessed physiologically by compound muscle action potential (CMAP), and the regenerated nerve tissues were evaluated histopathologically at 13 weeks after surgery.
Results
Facial nucleus stained with retrograde tracers proved the re‐innervation of affected facial muscle by the babysitter procedure. In CMAP, the amplitude of babysitter group was significantly higher than that of the cross‐facial nerve graft group (p |
| doi_str_mv | 10.1002/micr.30580 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2385274303</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2385274303</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3570-5bff741bdb1459adb30b0ba0c88db00efbcf3f3d699c4381ab79135f9923d5523</originalsourceid><addsrcrecordid>eNp90T1v1TAUBmALUdFLYeEHIEssqFLKsR039oiuSqnUqhWC2fLHMU2Vj4udtMrGysZv5JeQ3BQGBiYP5zmvjvUS8orBCQPg79rapxMBUsETsmGgVcEryZ-SDVRCFQyUPCTPc74DAK0r_YwcCs6FgLLckB83Ce-xG-q-o32kATtM93bAQNsx-wapHVK_u53oQz3cUus9Npj28wXir-8_E35dluw-wk3UWTflehgw0V3qPYYxIa07Ogsara9ts67SnU22mWmmbR-weUEOom0yvnx8j8iXD2eftx-Ly-vzi-37y8ILWUEhXYxVyVxwrJTaBifAgbPglQoOAKPzUUQRTrX2pVDMukozIaPWXAQpuTgib9fc-bpvI-bBtHWev9XYDvsxGy6U5FUpQMz0zT_0rh9TN19neFmdVlorvajjVfnU55wwml2qW5smw8AsBZmlILMvaMavHyNH12L4S_80MgO2goe6wek_UebqYvtpDf0NN9yfNQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2476799893</pqid></control><display><type>article</type><title>Prevention of denervated muscle atrophy with accelerated nerve‐regeneration by babysitter procedure in rat facial nerve paralysis model</title><source>Wiley-Blackwell Read & Publish Collection</source><creator>Hashimoto, Kazuki ; Matsumine, Hajime ; Osaki, Hironobu ; Ueta, Yoshifumi ; Kamei, Wataru ; Shimizu, Mari ; Fujii, Kaori ; Niimi, Yosuke ; Miyata, Mariko ; Sakurai, Hiroyuki</creator><creatorcontrib>Hashimoto, Kazuki ; Matsumine, Hajime ; Osaki, Hironobu ; Ueta, Yoshifumi ; Kamei, Wataru ; Shimizu, Mari ; Fujii, Kaori ; Niimi, Yosuke ; Miyata, Mariko ; Sakurai, Hiroyuki</creatorcontrib><description>Purpose
The “babysitter” procedure is a reconstruction technique for facial nerve complete paralysis and uses the movement source from the healthy facial nerve with a cross‐nerve graft. First, an end‐to‐side neurorrhaphy is performed between the affected facial nerve trunk and hypoglossal nerve for continuously delivering stimuli to the mimetic muscles for preventing the atrophy of mimetic muscles. Despite favorable clinical results, histological and physiological mechanisms remain unknown. This study attempted to establish a model for the “babysitter” procedure and find its efficacy in rats with facial nerve complete paralysis.
Materials and Methods
A total of 16 Lewis rats were used and divided into 2 groups; cross nerve graft (n = 8) and babysitter groups (n = 8). The facial nerve trunk was transected in both groups. Babysitter group underwent a two‐stage procedure. Cross nerve graft group underwent only the transfer of nerve graft from the healthy side to affected side. The animals were assessed physiologically by compound muscle action potential (CMAP), and the regenerated nerve tissues were evaluated histopathologically at 13 weeks after surgery.
Results
Facial nucleus stained with retrograde tracers proved the re‐innervation of affected facial muscle by the babysitter procedure. In CMAP, the amplitude of babysitter group was significantly higher than that of the cross‐facial nerve graft group (p < .05). Histological examination found a significant difference in myelin g‐ratio between two groups (p < .05).
Conclusion
This study investigated the “babysitter” procedure for rat facial nerve palsy. Babysitter procedure shortened the denervation period without mimic muscle atrophy.</description><identifier>ISSN: 0738-1085</identifier><identifier>EISSN: 1098-2752</identifier><identifier>DOI: 10.1002/micr.30580</identifier><identifier>PMID: 32233044</identifier><language>eng</language><publisher>Hoboken, USA: John Wiley & Sons, Inc</publisher><subject>Action potential ; Atrophy ; Denervation ; Facial nerve ; Grafting ; Grafts ; Hypoglossal nerve ; Innervation ; Muscles ; Myelin ; Nervous tissues ; Neural cell transplants ; Paralysis ; Regeneration ; Surgery ; Tracers</subject><ispartof>Microsurgery, 2021-01, Vol.41 (1), p.61-69</ispartof><rights>2020 Wiley Periodicals, Inc.</rights><rights>2021 Wiley Periodicals LLC.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3570-5bff741bdb1459adb30b0ba0c88db00efbcf3f3d699c4381ab79135f9923d5523</citedby><cites>FETCH-LOGICAL-c3570-5bff741bdb1459adb30b0ba0c88db00efbcf3f3d699c4381ab79135f9923d5523</cites><orcidid>0000-0001-6158-1169 ; 0000-0002-8997-755X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32233044$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hashimoto, Kazuki</creatorcontrib><creatorcontrib>Matsumine, Hajime</creatorcontrib><creatorcontrib>Osaki, Hironobu</creatorcontrib><creatorcontrib>Ueta, Yoshifumi</creatorcontrib><creatorcontrib>Kamei, Wataru</creatorcontrib><creatorcontrib>Shimizu, Mari</creatorcontrib><creatorcontrib>Fujii, Kaori</creatorcontrib><creatorcontrib>Niimi, Yosuke</creatorcontrib><creatorcontrib>Miyata, Mariko</creatorcontrib><creatorcontrib>Sakurai, Hiroyuki</creatorcontrib><title>Prevention of denervated muscle atrophy with accelerated nerve‐regeneration by babysitter procedure in rat facial nerve paralysis model</title><title>Microsurgery</title><addtitle>Microsurgery</addtitle><description>Purpose
The “babysitter” procedure is a reconstruction technique for facial nerve complete paralysis and uses the movement source from the healthy facial nerve with a cross‐nerve graft. First, an end‐to‐side neurorrhaphy is performed between the affected facial nerve trunk and hypoglossal nerve for continuously delivering stimuli to the mimetic muscles for preventing the atrophy of mimetic muscles. Despite favorable clinical results, histological and physiological mechanisms remain unknown. This study attempted to establish a model for the “babysitter” procedure and find its efficacy in rats with facial nerve complete paralysis.
Materials and Methods
A total of 16 Lewis rats were used and divided into 2 groups; cross nerve graft (n = 8) and babysitter groups (n = 8). The facial nerve trunk was transected in both groups. Babysitter group underwent a two‐stage procedure. Cross nerve graft group underwent only the transfer of nerve graft from the healthy side to affected side. The animals were assessed physiologically by compound muscle action potential (CMAP), and the regenerated nerve tissues were evaluated histopathologically at 13 weeks after surgery.
Results
Facial nucleus stained with retrograde tracers proved the re‐innervation of affected facial muscle by the babysitter procedure. In CMAP, the amplitude of babysitter group was significantly higher than that of the cross‐facial nerve graft group (p < .05). Histological examination found a significant difference in myelin g‐ratio between two groups (p < .05).
Conclusion
This study investigated the “babysitter” procedure for rat facial nerve palsy. Babysitter procedure shortened the denervation period without mimic muscle atrophy.</description><subject>Action potential</subject><subject>Atrophy</subject><subject>Denervation</subject><subject>Facial nerve</subject><subject>Grafting</subject><subject>Grafts</subject><subject>Hypoglossal nerve</subject><subject>Innervation</subject><subject>Muscles</subject><subject>Myelin</subject><subject>Nervous tissues</subject><subject>Neural cell transplants</subject><subject>Paralysis</subject><subject>Regeneration</subject><subject>Surgery</subject><subject>Tracers</subject><issn>0738-1085</issn><issn>1098-2752</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp90T1v1TAUBmALUdFLYeEHIEssqFLKsR039oiuSqnUqhWC2fLHMU2Vj4udtMrGysZv5JeQ3BQGBiYP5zmvjvUS8orBCQPg79rapxMBUsETsmGgVcEryZ-SDVRCFQyUPCTPc74DAK0r_YwcCs6FgLLckB83Ce-xG-q-o32kATtM93bAQNsx-wapHVK_u53oQz3cUus9Npj28wXir-8_E35dluw-wk3UWTflehgw0V3qPYYxIa07Ogsara9ts67SnU22mWmmbR-weUEOom0yvnx8j8iXD2eftx-Ly-vzi-37y8ILWUEhXYxVyVxwrJTaBifAgbPglQoOAKPzUUQRTrX2pVDMukozIaPWXAQpuTgib9fc-bpvI-bBtHWev9XYDvsxGy6U5FUpQMz0zT_0rh9TN19neFmdVlorvajjVfnU55wwml2qW5smw8AsBZmlILMvaMavHyNH12L4S_80MgO2goe6wek_UebqYvtpDf0NN9yfNQ</recordid><startdate>202101</startdate><enddate>202101</enddate><creator>Hashimoto, Kazuki</creator><creator>Matsumine, Hajime</creator><creator>Osaki, Hironobu</creator><creator>Ueta, Yoshifumi</creator><creator>Kamei, Wataru</creator><creator>Shimizu, Mari</creator><creator>Fujii, Kaori</creator><creator>Niimi, Yosuke</creator><creator>Miyata, Mariko</creator><creator>Sakurai, Hiroyuki</creator><general>John Wiley & Sons, Inc</general><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T5</scope><scope>7T7</scope><scope>7TK</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-6158-1169</orcidid><orcidid>https://orcid.org/0000-0002-8997-755X</orcidid></search><sort><creationdate>202101</creationdate><title>Prevention of denervated muscle atrophy with accelerated nerve‐regeneration by babysitter procedure in rat facial nerve paralysis model</title><author>Hashimoto, Kazuki ; Matsumine, Hajime ; Osaki, Hironobu ; Ueta, Yoshifumi ; Kamei, Wataru ; Shimizu, Mari ; Fujii, Kaori ; Niimi, Yosuke ; Miyata, Mariko ; Sakurai, Hiroyuki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3570-5bff741bdb1459adb30b0ba0c88db00efbcf3f3d699c4381ab79135f9923d5523</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Action potential</topic><topic>Atrophy</topic><topic>Denervation</topic><topic>Facial nerve</topic><topic>Grafting</topic><topic>Grafts</topic><topic>Hypoglossal nerve</topic><topic>Innervation</topic><topic>Muscles</topic><topic>Myelin</topic><topic>Nervous tissues</topic><topic>Neural cell transplants</topic><topic>Paralysis</topic><topic>Regeneration</topic><topic>Surgery</topic><topic>Tracers</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hashimoto, Kazuki</creatorcontrib><creatorcontrib>Matsumine, Hajime</creatorcontrib><creatorcontrib>Osaki, Hironobu</creatorcontrib><creatorcontrib>Ueta, Yoshifumi</creatorcontrib><creatorcontrib>Kamei, Wataru</creatorcontrib><creatorcontrib>Shimizu, Mari</creatorcontrib><creatorcontrib>Fujii, Kaori</creatorcontrib><creatorcontrib>Niimi, Yosuke</creatorcontrib><creatorcontrib>Miyata, Mariko</creatorcontrib><creatorcontrib>Sakurai, Hiroyuki</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Microsurgery</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hashimoto, Kazuki</au><au>Matsumine, Hajime</au><au>Osaki, Hironobu</au><au>Ueta, Yoshifumi</au><au>Kamei, Wataru</au><au>Shimizu, Mari</au><au>Fujii, Kaori</au><au>Niimi, Yosuke</au><au>Miyata, Mariko</au><au>Sakurai, Hiroyuki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Prevention of denervated muscle atrophy with accelerated nerve‐regeneration by babysitter procedure in rat facial nerve paralysis model</atitle><jtitle>Microsurgery</jtitle><addtitle>Microsurgery</addtitle><date>2021-01</date><risdate>2021</risdate><volume>41</volume><issue>1</issue><spage>61</spage><epage>69</epage><pages>61-69</pages><issn>0738-1085</issn><eissn>1098-2752</eissn><abstract>Purpose
The “babysitter” procedure is a reconstruction technique for facial nerve complete paralysis and uses the movement source from the healthy facial nerve with a cross‐nerve graft. First, an end‐to‐side neurorrhaphy is performed between the affected facial nerve trunk and hypoglossal nerve for continuously delivering stimuli to the mimetic muscles for preventing the atrophy of mimetic muscles. Despite favorable clinical results, histological and physiological mechanisms remain unknown. This study attempted to establish a model for the “babysitter” procedure and find its efficacy in rats with facial nerve complete paralysis.
Materials and Methods
A total of 16 Lewis rats were used and divided into 2 groups; cross nerve graft (n = 8) and babysitter groups (n = 8). The facial nerve trunk was transected in both groups. Babysitter group underwent a two‐stage procedure. Cross nerve graft group underwent only the transfer of nerve graft from the healthy side to affected side. The animals were assessed physiologically by compound muscle action potential (CMAP), and the regenerated nerve tissues were evaluated histopathologically at 13 weeks after surgery.
Results
Facial nucleus stained with retrograde tracers proved the re‐innervation of affected facial muscle by the babysitter procedure. In CMAP, the amplitude of babysitter group was significantly higher than that of the cross‐facial nerve graft group (p < .05). Histological examination found a significant difference in myelin g‐ratio between two groups (p < .05).
Conclusion
This study investigated the “babysitter” procedure for rat facial nerve palsy. Babysitter procedure shortened the denervation period without mimic muscle atrophy.</abstract><cop>Hoboken, USA</cop><pub>John Wiley & Sons, Inc</pub><pmid>32233044</pmid><doi>10.1002/micr.30580</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0001-6158-1169</orcidid><orcidid>https://orcid.org/0000-0002-8997-755X</orcidid></addata></record> |
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| ispartof | Microsurgery, 2021-01, Vol.41 (1), p.61-69 |
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| language | eng |
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| source | Wiley-Blackwell Read & Publish Collection |
| subjects | Action potential Atrophy Denervation Facial nerve Grafting Grafts Hypoglossal nerve Innervation Muscles Myelin Nervous tissues Neural cell transplants Paralysis Regeneration Surgery Tracers |
| title | Prevention of denervated muscle atrophy with accelerated nerve‐regeneration by babysitter procedure in rat facial nerve paralysis model |
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