Preparation of complex microcapsules of soluble polysaccharide from Glycyrrhiza uralensis and its application in wound repair and scar inhibition

• Published in: International journal of biological macromolecules 2020-08, Vol.156, p.906-917
• Main Authors: Hao, Baocheng, Wang, Xinjian, Ma, Xueqing, Jin, Yongming, Fan, Wenguang, Laba, Cidan, Wujin, Cuomu, Wang, Yonggang, Liang, Jianping
• Format: Article
• Language: English
• Subjects: Animals; Capsules - chemistry; Capsules - therapeutic use; Chemical Phenomena; Glycyrrhiza polysaccharide; Glycyrrhiza uralensis - chemistry; Macromolecular Substances - chemistry; Macromolecular Substances - therapeutic use; Microcapsule; Models, Animal; Neovascularization, Physiologic; Polysaccharides - chemistry; Rats; Solubility; Spectrum Analysis; Wound Healing
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2020.03.121

The extraction process of Glycyrrhiza soluble polysaccharide (GP) was optimized by RSM, a rat trauma model was established via longitudinal incision on the back skin. The effects of GP combined with microcapsule collagen on the repair of rat injury model were discussed at different levels, Based on the content of hydroxyproline at the whole animal level, the proliferation of granulation tissue stained by HE, the number of microvessels labeled by CD34, the production of collagen fibers stained by Masson, the level of phosphorylation of STAT3 protein and that of VEGF at protein level were investigated. The results showed that after the administration of GP combined with microcapsules, the content of hydroxyproline in granulation tissue increased, the proliferation of capillaries and fibroblasts in granulation tissue became active, and the number of microvessels in wound increased. The formation density of collagen fibers was uniform and orderly. GP combined with microcapsules could activate the expression of p-STAT3 and VEGF proteins and up-regulate the transcription level of VEGF mRNA and miRNA-21 genes. Furthermore, GP combined with microcapsules could accelerate wound healing and promote neovascularization. •The extraction process of Glycyrrhiza soluble polysaccharide (GP) was optimized by RSM.•The effects of GP combined with microcapsule (GPM) on the repair of rat injury model were discussed at different levels.•GPM could accelerate wound healing and promote neovascularization.