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Long non-coding RNA CRNDE deteriorates intrauterine infection-induced neonatal brain injury
This study aimed to test the hypothesis that long non-coding RNA (lncRNA) colorectal neoplasia differentially expressed (CRNDE) could exacerbate brain injury caused by intrauterine infection in neonatal rats. Intrauterine infection was induced in pregnant rats by lipopolysaccharide (LPS). After deli...
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Published in: | Molecular and cellular probes 2020-08, Vol.52, p.101565-101565, Article 101565 |
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Main Authors: | , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | This study aimed to test the hypothesis that long non-coding RNA (lncRNA) colorectal neoplasia differentially expressed (CRNDE) could exacerbate brain injury caused by intrauterine infection in neonatal rats.
Intrauterine infection was induced in pregnant rats by lipopolysaccharide (LPS). After delivery, newborn rats with brain injury caused by intrauterine infection were randomly divided into control, control shRNA, and CRNDE shRNA groups. CRNDE expression in serum and amniotic fluid of pregnant rats and neonatal brain tissues were determined by quantitative real-time PCR (qRT-PCR). Morris water maze (MWM) task was used to test the spatial learning and memory ability. Histological examination and apoptosis detection were performed by hematoxylin and eosin (H&E) and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining, respectively. Immunohistochemistry was conducted to evaluate the activation of astrocytes and microglia.
LncRNA CRNDE was highly expressed in serum and amniotic fluid of maternal rats and in brain tissues of offspring rats. Furthermore, shRNA-mediated CRNDE downregulation could rescue the spatial learning and memory ability, improve brain histopathological changes and cell death, and inhibit the activation of astrocytes and microglia caused by LPS.
CRNDE silencing possessed a cerebral protective effect in neonatal rats with brain injury caused by interauterine infection.
•CRNDE was upregulated in serum and amniotic fluid of maternal rats.•CRNDE was upregulated in neonatal rats with intrauterine infection.•CRNDE knockdown ameliorated LPS-induced brain injury in offspring rats. |
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ISSN: | 0890-8508 1096-1194 |
DOI: | 10.1016/j.mcp.2020.101565 |