Morphological redescription and phylogenetic assessment of Spauligodon aspiculus (Oxyuroidea: Pharyngodonidae) infecting the white‐spotted gecko, Tarentola annularis (Squamata: Gekkonidae)

• Published in: Microscopy research and technique 2020-08, Vol.83 (8), p.901-919
• Main Authors: Abdel‐Ghaffar, Fathy, Varjabedian, Kohar G., Fol, Mona, Talal, Noha, Abdel‐Gaber, Rewaida, Al Quraishy, Saleh
• Format: Article
• Language: English
• Subjects: Animals; Congeners; Eggs; Egypt; Female; Geckos; Gekkonidae; Intestine, Small - parasitology; Lizards - parasitology; Male; Molecular Typing; Morphology; Nematodes; Oxyuroidea - anatomy & histology; Oxyuroidea - classification; Oxyuroidea - genetics; Papillae; Parasites; Parasitic diseases; Phylogenetics; Phylogeny; Physical characteristics; RNA, Ribosomal, 28S - genetics; rRNA 28S; Spauligodon aspiculus; Species; Tarentola annularis; Taxonomy; Vulva; Worms
• ISSN: 1059-910X; 1097-0029
• DOI: 10.1002/jemt.23484

Thirty white‐spotted geckos, Tarentola annularis, from the South Sinai desert in Egypt, were examined for helminth parasites. Spauligodon aspiculus was observed to infect 19 geckos with 63.33% as a prevalence of parasitic infection. The present nematode species is separated from congeners by morphological and metrical characteristics such as lateral alae, aspinose filamentous tail, and no spicule, and three pairs of caudal papillae with posterior pair excluded from envelopment by the caudal alae in the male worms, and knobbed eggs, and postbulbar vulva in females. It compared morphometrically with other Spauligodon species described previously and showed few differences in measurements. Molecular characterization based on the partial 28S rRNA nuclear ribosomal gene sequence showed that there was a close identity, up to 72%, with other sequences retrieved from GenBank. Phylogenetic analysis showed that the parasite sequence in conjunction with existing data facilitates the investigation of the placement of this pharyngodonid species within Oxyuridae. The present species is deeply embedded in the genus Spauligodon with close relationships to previously described Spauligodon nicolauensis (gb| JN619349.1, and JF829243.1) as more related sister taxa. This study highlights the importance of combining genetic and morphological data with taxonomy in pharyngodonid species. The present study represents: the second report for describing Spauligodon aspiculus infecting the white‐spotted gecko, Tarentola annularis, in Egypt; the first molecular phylogenetic study demonstrating unique genetic sequences for this Spauligodon species.