A SNP panel for early detection of artificial chimerism in HSCT patients using TaqMan technology

The monitoring of chimerism status in a hematopoietic stem cell transplantation patient is a crucial process and is performed periodically in a short time interval. A short tandem repeat marker is widely used for chimerism analysis due to its high discrimination power. However, the sensitivity of th...

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Bibliographic Details
Published in:International journal of legal medicine 2020-09, Vol.134 (5), p.1553-1561
Main Authors: Sathirapatya, Tikumphorn, Worrapitirungsi, Wikanda, Sukawutthiya, Poonyapat, Rasmeepaisarn, Kawin, Vongpaisarnsin, Kornkiat
Format: Article
Language:English
Subjects:
Asian Continental Ancestry Group - ethnology
Chimerism
Forensic Medicine
Genealogy
Genotype & phenotype
Genotyping Techniques
Hematopoietic Stem Cell Transplantation - ethnology
Humans
Markers
Medical Law
Medical technology
Medicine
Medicine & Public Health
Microsatellite Repeats
Monitoring, Physiologic
Original Article
Polymorphism, Single Nucleotide
Real-Time Polymerase Chain Reaction
Sensitivity and Specificity
Stem cell transplantation
Stem cells
Technology assessment
Technology utilization
Transplantation
Transplants & implants
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Summary:The monitoring of chimerism status in a hematopoietic stem cell transplantation patient is a crucial process and is performed periodically in a short time interval. A short tandem repeat marker is widely used for chimerism analysis due to its high discrimination power. However, the sensitivity of this approach was limited to 5% of a minor contributor and the interpretation is usually interrupted with PCR stochastic phenomena. Here, we developed an SNP panel for chimerism analysis using TaqMan technology. A set of SNPs was selected from Thai ancestry informative markers and open-access databases with proper criteria. We examined the 30 recipient-donor pairs that underwent HSCT and showed that the panel can provide an informative marker from 90% of all pairs. An early detection of artificial chimerism in post-HSCT samples was observed when compared with STR analysis. In addition, the detail of cases was discussed.
ISSN:0937-9827
1437-1596
DOI:10.1007/s00414-020-02276-2