DHRS12 inhibits the proliferation and metastasis of osteosarcoma via Wnt3a/β-catenin pathway

• Published in: Future oncology (London, England) England), 2020-04, Vol.16 (11), p.665-674
• Main Authors: Xu, Zhixian, He, Wubing, Ke, Tie, Zhang, Yongfa, Zhang, Guifeng
• Format: Article
• Language: English
• Subjects: Animals; Antibodies; Apoptosis; beta Catenin - metabolism; Bone cancer; Cell culture; Cell growth; Cell Line, Tumor; Cell Movement; Cell Proliferation; Chemotherapy; Data analysis; DHRS12; Down-Regulation; Gene expression; Gene Expression Regulation, Neoplastic; Humans; Hypoxia; invasion; Metastasis; Mice; Neoplasm Invasiveness; Neoplasm Metastasis; Neoplasm Transplantation; osteosarcoma; Osteosarcoma - genetics; Osteosarcoma - metabolism; Osteosarcoma - mortality; Osteosarcoma - pathology; Pathogenesis; Plasmids; proliferation; Proteins; Sarcoma; Short Chain Dehydrogenase-Reductases - genetics; Short Chain Dehydrogenase-Reductases - metabolism; Signal transduction; Statistical analysis; Survival Rate; Tumors; Wnt Signaling Pathway; Wnt3A Protein - metabolism; Wnt3a/β-catenin
• ISSN: 1479-6694; 1744-8301
• DOI: 10.2217/fon-2019-0432

This experimental design was based on DHRS12 to explore its biological effects on osteosarcoma (OS). The expression level of endogenous DHRS12 was analyzed by immunohistochemical analysis. DHRS12 was overexpressed in MG-63 and HOS cells by plasmid transfection. Cell proliferation, invasion, migration, apoptosis and western blot were used in the experiment. The expression of DHRS12 was significantly reduced in OS. Overexpression of DHRS12 inhibited the proliferation, migration and invasion of MG-63 and HOS cells and induced apoptosis of OS cells. Overexpression of DHRS12 upregulated Bax, Caspase 9 and Caspase 3. Overexpression of DHRS12 resulted in inactivation of the Wnt3a/β-catenin signaling pathway. Overexpression of DHRS12 inhibited the progression of OS via the Wnt3a/β-catenin pathway.