An Early Fecal Microbiota Transfer Improves the Intestinal Conditions on Microflora and Immunoglobulin and Antimicrobial Peptides in Piglets

• Published in: Journal of agricultural and food chemistry 2020-04, Vol.68 (17), p.4830-4843
• Main Authors: Teng, Teng, Gao, Feng, He, Wei, Fu, Huiyang, Guo, Jing, Bai, Guangdong, Shi, Baoming
• Format: Article
• Language: English
• Subjects: Animals; Animals, Newborn - immunology; Animals, Newborn - microbiology; Fecal Microbiota Transplantation; Feces - microbiology; Female; Gastrointestinal Microbiome; Immunoglobulin G - immunology; Intestinal Mucosa - immunology; Intestinal Mucosa - microbiology; Jejunum - immunology; Jejunum - microbiology; Male; Pore Forming Cytotoxic Proteins - immunology; Swine
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/acs.jafc.0c00545

The goal of this study was to investigate the effects of early fecal microbial transfer (FMT) on the microflora of recipient piglets, where Yorkshire newborn piglets and Min sows (an indigenous pig breed in China) were used as the fecal recipients and donors, respectively, to reveal the changes in immunity and development-related functions of the intestinal mucosa driven by FMT. The recipient group was inoculated with fecal microbial fluids from days 1 to 10. On day 21, the relative abundance of the Proteobacteria was reduced; the concentrations of immunoglobulin M (IgM) and immunoglobulin G (IgG) in the jejunal mucosa, and that of IgG in the ileal mucosa of the recipient group, were increased (P < 0.05). On day 40, the relative abundance of the Firmicutes in the recipient group was increased, while that of Bacteroides was decreased. The concentrations of IgG and IgM in the ileal mucosa of the recipient group were increased. FMT protected the intestine by modulating the antimicrobial peptides of the intestinal mucosa (P < 0.05). The results of this study revealed that early FMT can improve the gut microbiota, intestinal mucosal immunity, and intestinal development-related functions of Yorkshire piglets.